Anti-Trophoblast specific protein alpha antibody (ab104401)
Key features and details
- Rabbit polyclonal to Trophoblast specific protein alpha
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Trophoblast specific protein alpha antibody -
Description
Rabbit polyclonal to Trophoblast specific protein alpha -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Mouse Trophoblast specific protein alpha aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab117405) -
Positive control
- This antibody gave a positive signal in Mouse trophoblast tissue lysate as well as the following whole cell lysates: MEF1; NIH3T3; STO.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab104401 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 1 µg/ml. WB Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. Target
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Relevance
Trophoblast specific protein alpha is a marker of spongiotrophoblasts. -
Database links
- Entrez Gene: 21984 Mouse
- SwissProt: P13438 Mouse
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Alternative names
- Tb1 antibody
- Tpbp antibody
- Tpbpa antibody
see all
Images
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Western blot - Anti-Trophoblast specific protein alpha antibody (ab104401)All lanes : Anti-Trophoblast specific protein alpha antibody (ab104401) at 1 µg/ml
Lane 1 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Trophoblast Stem Cells (Mouse) Whole Cell Lysate
Lane 4 : STO (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Additional bands at: 150 kDa, 255 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 14 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Trophoblast specific protein alpha antibody (ab104401)ICC/IF image of ab104401 stained MEF1 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96899, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab104401, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MEF1 cells at 1µg/ml.
Protocols
Datasheets and documents
References (12)
ab104401 has been referenced in 12 publications.
- Peng K et al. Derivation of Haploid Trophoblast Stem Cells via Conversion In Vitro. iScience 11:508-518 (2019). PubMed: 30635215
- Wattez JS et al. The platelet-derived growth factor receptor alpha promoter-directed expression of cre recombinase in mouse placenta. Dev Dyn 248:363-374 (2019). PubMed: 30843624
- Sharma A et al. miR-126 regulates glycogen trophoblast proliferation and DNA methylation in the murine placenta. Dev Biol 449:21-34 (2019). PubMed: 30771304
- Segura-Bayona S et al. Differential requirements for Tousled-like kinases 1 and 2 in mammalian development. Cell Death Differ 24:1872-1885 (2017). PubMed: 28708136
- Monsivais D et al. BMP7 Induces Uterine Receptivity and Blastocyst Attachment. Endocrinology 158:979-992 (2017). PubMed: 28324064
Images
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Western blot - Anti-Trophoblast specific protein alpha antibody (ab104401)All lanes : Anti-Trophoblast specific protein alpha antibody (ab104401) at 1 µg/ml
Lane 1 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Trophoblast Stem Cells (Mouse) Whole Cell Lysate
Lane 4 : STO (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Additional bands at: 150 kDa, 255 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 14 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-Trophoblast specific protein alpha antibody (ab104401)ICC/IF image of ab104401 stained MEF1 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96899, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab104401, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MEF1 cells at 1µg/ml.