Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1104(2)] to TrkA + TrkB
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-TrkA + TrkB antibody [EPR1104(2)]

  • Description

    Rabbit monoclonal [EPR1104(2)] to TrkA + TrkB

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal brain and SH SY5Y lysates; Human brain tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)
    Western blot - Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)
    All lanes : Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010) at 1.118 µg/ml (purified)

    Lanes 1 & 3 & 5 : Empty vector overexpression 293T cell lysates
    Lane 2 : His-Human TrkC overexpression 293T cell lysates
    Lane 4 : His-Human TrkA overexpression 293T cell lysates
    Lane 6 : His-Human TrkB overexpression 293T cell lysates
    Lane 7 : Human brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

    Predicted band size: 87 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting Buffer and concentration: 5% NFDM /TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)

    ab109010 at 1/250 dilution staining TrkA + TrkB in Human brain by Immunohistochemistry, Paraffin-embedded tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Western blot - Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)
    Western blot - Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)
    All lanes : Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : SH SY5Y lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 87 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)

    ab109010 showing negative staining in Normal human kidney tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)

    ab109010 showing negative staining in Normal human liver tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)

    ab109010 showing negative staining in Normal human tonsil tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)

    ab109010 showing negative staining in Normal human colon tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)
    Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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