Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸

Order now and get it on Thursday March 04, 2021

Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1104(2)] to TrkA + TrkB
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-TrkA + TrkB antibody [EPR1104(2)]
Description

Rabbit monoclonal [EPR1104(2)] to TrkA + TrkB
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal brain and SH SY5Y lysates; Human brain tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR1104(2)
Isotype

IgG
Research areas

Western blot - Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)

All lanes : Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010) at 1.118 µg/ml (purified)

Lanes 1 & 3 & 5 : Empty vector overexpression 293T cell lysates
Lane 2 : His-Human TrkC overexpression 293T cell lysates
Lane 4 : His-Human TrkA overexpression 293T cell lysates
Lane 6 : His-Human TrkB overexpression 293T cell lysates
Lane 7 : Human brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

Predicted band size: 87 kDa
Observed band size: 120 kDa
why is the actual band size different from the predicted?

Blocking/Diluting Buffer and concentration: 5% NFDM /TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)

ab109010 at 1/250 dilution staining TrkA + TrkB in Human brain by Immunohistochemistry, Paraffin-embedded tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Western blot - Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)

All lanes : Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : SH SY5Y lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 87 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)

ab109010 showing negative staining in Normal human kidney tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)

ab109010 showing negative staining in Normal human liver tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)

ab109010 showing negative staining in Normal human tonsil tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)

ab109010 showing negative staining in Normal human colon tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com