Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Transferrin Receptor antibody [EPR4012] - BSA and Azide free (ab271878)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4012] to Transferrin Receptor - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Transferrin Receptor antibody [EPR4012] - BSA and Azide free
    See all Transferrin Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR4012] to Transferrin Receptor - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human placenta, TF-1, HeLa and A549 cell lysates

  • General notes

    ab271878 is the carrier-free version of ab108985. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-Transferrin Receptor antibody [EPR4012] - BSA and Azide free (ab271878)
    Flow Cytometry - Anti-Transferrin Receptor antibody [EPR4012] - BSA and Azide free (ab271878)

    Overlay histogram showing Jurkat cells stained with ab108985 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108985, 1/100) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108985).

  • Anti-Transferrin Receptor antibody [EPR4012] - BSA and Azide free (ab271878)
    Anti-Transferrin Receptor antibody [EPR4012] - BSA and Azide free (ab271878)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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