Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Transferrin Receptor antibody [EPR20584] - BSA and Azide free (ab232376)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20584] to Transferrin Receptor - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Transferrin Receptor antibody [EPR20584] - BSA and Azide free
    See all Transferrin Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR20584] to Transferrin Receptor - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human placenta tissue.

  • General notes

    Ab232376 is the carrier-free version of ab214039. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232376 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-Transferrin Receptor antibody [EPR20584] - BSA and Azide free (ab232376)
    Immunoprecipitation - Anti-Transferrin Receptor antibody [EPR20584] - BSA and Azide free (ab232376)

    Transferrin Receptor was immunoprecipitated from 0.35 mg of K562 (human chronic myelogenous leukemia cell line from bone marrow) lysate with ab214039 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab214039 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: K562 whole cell lysate 10 µg (Input). 

    Lane 2: ab214039 IP in K562 whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab214039 in K562 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214039).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [EPR20584] - BSA and Azide free (ab232376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [EPR20584] - BSA and Azide free (ab232376)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Transferrin Receptor with ab214039 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on renal tubules of mouse kidney (PMID: 12538733). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214039).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [EPR20584] - BSA and Azide free (ab232376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [EPR20584] - BSA and Azide free (ab232376)

    Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue labeling Transferrin Receptor with ab214039 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on human esophageal cancer. The staining intensity of human esophageal cancer tissue was stronger than the human paracarcinoma esophagus tissue. Both paracarcinoma and human esophageal cancer tissues have been taken from the same patient (PMID: 24435655). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214039).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [EPR20584] - BSA and Azide free (ab232376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [EPR20584] - BSA and Azide free (ab232376)

    Immunohistochemical analysis of paraffin-embedded human esophagus tissue labeling Transferrin Receptor with ab214039 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Weakly cytoplasmic staining on human paracarcinoma esophagus. The staining intensity of human paracarcinoma esophagus was weaker than the human esophageal cancer tissue. Both paracarcinoma and human esophageal cancer tissues have been taken from the same patient (PMID: 24435655). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214039).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [EPR20584] - BSA and Azide free (ab232376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [EPR20584] - BSA and Azide free (ab232376)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Transferrin Receptor with ab214039 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on capillaries of human cerebrum (PMID: 6095085). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214039).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [EPR20584] - BSA and Azide free (ab232376)
    Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [EPR20584] - BSA and Azide free (ab232376)

    Immunofluorescent analysis of 100% methanol-fixed RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling Transferrin Receptor with ab214039 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing positive staining on RAW 264.7 cells.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214039).

  • Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [EPR20584] - BSA and Azide free (ab232376)
    Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [EPR20584] - BSA and Azide free (ab232376)

    Immunofluorescent analysis of 100% methanol-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling Transferrin Receptor with ab214039  at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing positive staining on K562 cells.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214039).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [EPR20584] - BSA and Azide free (ab232376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [EPR20584] - BSA and Azide free (ab232376)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Transferrin Receptor with ab214039 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on human placenta (PMID: 27483296). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214039).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

  • Anti-Transferrin Receptor antibody [EPR20584] - BSA and Azide free (ab232376)
    Anti-Transferrin Receptor antibody [EPR20584] - BSA and Azide free (ab232376)

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