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Anti-Transferrin Receptor antibody [8D3] - BSA and Azide free (ab256572)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [8D3] to Transferrin Receptor - BSA and Azide free
  • Suitable for: ICC, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Transferrin Receptor antibody [8D3] - BSA and Azide free
    See all Transferrin Receptor primary antibodies

  • Description

    Rat monoclonal [8D3] to Transferrin Receptor - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC
    Mouse
    See all applications and species data

  • Immunogen

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: A20 cells. Flow Cyt: A20 cells.

  • General notes

    ab256572 is the carrier-free version of ab256568. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256572 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunocytochemistry - Anti-Transferrin Receptor antibody [8D3] - BSA and Azide free (ab256572)
    Immunocytochemistry - Anti-Transferrin Receptor antibody [8D3] - BSA and Azide free (ab256572)

    This data was produced using the same antibody clone but in a different buffer formulation, ab256568.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A20 (mouse reticulum sarcoma B lymphocyte) cells labelling Transferrin Receptor with ab256568 at 1/50 dilution, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous and cytoplasmic staining in A20 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue). 

    -ve control 1: ab256568 at 1/50 dilution followed by ab150080 at 1/500 dilution.

    -ve control 2: ab179513 at 1/200 dilution followed by ab150157 at 1/1000 dilution.

  • Flow Cytometry - Anti-Transferrin Receptor antibody [8D3] - BSA and Azide free (ab256572)
    Flow Cytometry - Anti-Transferrin Receptor antibody [8D3] - BSA and Azide free (ab256572)

    This data was produced using the same antibody clone but in a different buffer formulation, ab256568.

    Flow cytometric analysis of A20 (mouse reticulum sarcoma B lymphocyte) cells labelling Transferrin Receptor with ab256568 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • Anti-Transferrin Receptor antibody [8D3] - BSA and Azide free (ab256572)
    Anti-Transferrin Receptor antibody [8D3] - BSA and Azide free (ab256572)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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