Anti-TPX2 antibody (ab264124)
Key features and details
- Rabbit polyclonal to TPX2
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TPX2 antibody
See all TPX2 primary antibodies -
Description
Rabbit polyclonal to TPX2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human TPX2 aa 150-200. The exact sequence is proprietary.
Database link: Q9ULW0 -
Positive control
- ICC/IF: Asynchronous HeLa cell. WB: HeLa whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-TPX2 antibody (ab264124)
Formaldehyde-fixed asynchronous HeLa (Human epithelial cell line from cervix adenocarcinoma) cell stained for TPX2 using ab264124 at a 1/400 dilution in ICC/IF. A red-fluorescent goat anti-rabbit IgG heavy and light chain, cross-adsorbed DyLight® 594 conjugated secondary antibody was used at a dilution of 1/100.
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Western blot - Anti-TPX2 antibody (ab264124)All lanes : Anti-TPX2 antibody (ab264124) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Predicted band size: 86 kDa
Exposure time: 1 minute4-8% SDS-PAGE.
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Immunoprecipitation - Anti-TPX2 antibody (ab264124)TPX2 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264124 at 0.4µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264124 at 0.1µg/ml.
Lane 1: HeLa whole cell lysate 50µg (Input).
Lane 2: ab264124 IP in HeLa whole cell lysate.
Lane 3: ab264124 IP in HeLa whole cell lysate plus blocking peptide.Exposure time: 1 min.
