Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TNNI1 antibody [EPR17120-11] (ab203515)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17120-11] to TNNI1
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-TNNI1 antibody [EPR17120-11]
    See all TNNI1 primary antibodies

  • Description

    Rabbit monoclonal [EPR17120-11] to TNNI1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal heart and skeletal muscle lysates. IHC-P: Human skeletal muscle tissue. IP: Human fetal heart whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17120-11

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-TNNI1 antibody [EPR17120-11] (ab203515)
    Western blot - Anti-TNNI1 antibody [EPR17120-11] (ab203515)
    All lanes : Anti-TNNI1 antibody [EPR17120-11] (ab203515) at 1/2000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    TNNI1 is abundantly expressed in the human skeletal muscle and heart.

  • Western blot - Anti-TNNI1 antibody [EPR17120-11] (ab203515)
    Western blot - Anti-TNNI1 antibody [EPR17120-11] (ab203515)
    Anti-TNNI1 antibody [EPR17120-11] (ab203515) at 1/2000 dilution + Human skeletal muscle lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    TNNI1 is abundantly expressed in the human skeletal muscle and heart.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNI1 antibody [EPR17120-11] (ab203515)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNI1 antibody [EPR17120-11] (ab203515)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling TNNI1 with ab203515 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on striated Human skeletal muscle tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNI1 antibody [EPR17120-11] (ab203515)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNI1 antibody [EPR17120-11] (ab203515)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TNNI1 with ab203515 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. No staining on Human kidney tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-TNNI1 antibody [EPR17120-11] (ab203515)
    Immunoprecipitation - Anti-TNNI1 antibody [EPR17120-11] (ab203515)

    TNNI1 was immunoprecipitated from 1mg of Human fetal heart whole cell lysate with ab203515 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203515 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Human fetal heart whole cell lysate 10 µg (Input). Lane 2: ab203515 IP in Human fetal heart whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203515 in Human fetal heart whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Anti-TNNI1 antibody [EPR17120-11] (ab203515)
    Anti-TNNI1 antibody [EPR17120-11] (ab203515)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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