Anti-TMEM16A antibody [SP31] (ab64085)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP31] to TMEM16A
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-TMEM16A antibody [SP31]
See all TMEM16A primary antibodies -
Description
Rabbit monoclonal [SP31] to TMEM16A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P Human -
Immunogen
Synthetic peptide within Human TMEM16A aa 400-500. The exact sequence is proprietary.
Database link: Q5XXA6 -
Positive control
- Human GIST tumorFlow Cyt: PC-3 cells.
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General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
ab64085 was switched from a hybridoma to recombinant production method on 23rd July 2019.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SP31 -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of Human Gastrointestinal Stromal Tumor tissue labelling TMEM16A with ab64085.
This image was generated from the hybridoma version.
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Flow Cytometry analysis of PC-3 (Human prostate adenocarcinoma epithelial cell) cells labeling TMEM16A with purified ab64085. (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.
This image was generated from the hybridoma version.
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TMEM16A detected in paraffin-embedded sections of human submucosal glands from CF patients and control samples.
TMEM16A expression was modest in non-CF submucosal glands of non-CF samples (b) but markedly increased in tissues from CF patients (d), with a particularly strong signal (f) in histologically altered glands (e). Magnification: X630 in a, X400 in b-f. Images 4Aa and 4Ba,c,e show hematoxylin and eosin staining.
This image was generated from the hybridoma version.
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ab64085 showing expression of TMEM16A in human surface epithelium tissue. TMEM16A staining was mostly absent (b), and sometimes scanty (c) in the respiratory epithelium lining bronchi or bronchioles from CF patients (c, arrows). A weak expression was also detectable in microvessels of peri-bronchial connective tissue (b, arrows). Magnification: X400.
This image was generated from the hybridoma version.
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