Anti-CLNS1A/CLCI antibody - C-terminal (ab192907)
Key features and details
- Rabbit polyclonal to CLNS1A/CLCI - C-terminal
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CLNS1A/CLCI antibody - C-terminal
See all CLNS1A/CLCI primary antibodies -
Description
Rabbit polyclonal to CLNS1A/CLCI - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Goat, Horse, Cow, Dog, Pig, Xenopus laevis, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide within Human CLNS1A/CLCI aa 150 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_001284.1).
Database link: P54105 -
Positive control
- HeLa, 293T, Jurkat, TCMK-1 and NIH3T3 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab192907 was affinity purified using an epitope specific to CLNS1A/CLCI immobilized on solid support -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLNS1A/CLCI antibody - C-terminal (ab192907)
ab192907 staining CLNS1A/CLCI in mouse tetratoma tissue sections. Primary antibody was used at a dilution of 1/1000.
Counterstain: Hematoxylin (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLNS1A/CLCI antibody - C-terminal (ab192907)ab192907 staining CLNS1A/CLCI in human ovarian carcinoma tissue sections. Primary antibody used at a dilution of 1/1000.
Counterstain: Hematoxylin (blue).
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Western blot - Anti-CLNS1A/CLCI antibody - C-terminal (ab192907)All lanes : Anti-CLNS1A/CLCI antibody - C-terminal (ab192907) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 26 kDa
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-CLNS1A/CLCI antibody - C-terminal (ab192907)Detection of CLNS1A/CLCI in immunoprecipitates of 293T whole cell lysate prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab192907 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
