Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BAP1 antibody [EPR22826-65] - BSA and Azide free (ab256480)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22826-65] to BAP1 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-BAP1 antibody [EPR22826-65] - BSA and Azide free
    See all BAP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22826-65] to BAP1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, PC-3, HepG2, A375, MDA-MB-231 and A549 whole cell lysates. Flow Cyt: PC-3 cells. IHC: Rat and mouse cerebrum, Human lung and colon carcinoma.

  • General notes

    Ab256480 is the carrier-free version of ab255611. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256480 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] - BSA and Azide free (ab256480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] - BSA and Azide free (ab256480)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebrum tissue labelling BAP1 with ab255611 at 1/100 dilution. Heat mediated antigen retrieval was performed usingTris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody.  Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Nuclear staining on rat cerebrum. The section was incubated with ab255611 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255611).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] - BSA and Azide free (ab256480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] - BSA and Azide free (ab256480)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue labelling BAP1 with ab255611 at 1/100 dilution. Heat mediated antigen retrieval was performed usingTris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody.  Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Nuclear staining on mouse cerebrum. The section was incubated with ab255611 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255611).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] - BSA and Azide free (ab256480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] - BSA and Azide free (ab256480)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue labelling BAP1 with ab255611 at 1/100 dilution. Heat mediated antigen retrieval was performed usingTris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody.  Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Nuclear staining on human lung carcinoma. The section was incubated with ab255611 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255611).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] - BSA and Azide free (ab256480)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] - BSA and Azide free (ab256480)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue labelling BAP1 with ab255611 at 1/100 dilution. Heat mediated antigen retrieval was performed usingTris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody.  Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Nuclear staining on human colon carcinoma. The section was incubated with ab255611 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255611).

  • Flow Cytometry - Anti-BAP1 antibody [EPR22826-65] - BSA and Azide free (ab256480)
    Flow Cytometry - Anti-BAP1 antibody [EPR22826-65] - BSA and Azide free (ab256480)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labeling BAP1 with ab255611 at 1/60 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255611).

  • Anti-BAP1 antibody [EPR22826-65] - BSA and Azide free (ab256480)
    Anti-BAP1 antibody [EPR22826-65] - BSA and Azide free (ab256480)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-BAP1 antibody [EPR22826-65] - BSA and Azide free (ab256480)

© 2021 Astana Biomed Group LLP. All rights reserved.