Anti-TLS/FUS (methyl ) antibody [9G6] (ab252830)
![Anti-TLS/FUS (methyl ) antibody [9G6] (ab252830)](https://www.abcam.com/ps/products/252/ab252830/Images/ab252830-397818-anti-none-antibody-9g6-western-blot-wildtype-hap1-whole-cell-lysate.jpg "Anti-TLS/FUS (methyl ) antibody [9G6] (ab252830)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [9G6] to TLS/FUS (methyl )
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-TLS/FUS (methyl ) antibody [9G6]
See all TLS/FUS primary antibodies -
Description
Rat monoclonal [9G6] to TLS/FUS (methyl ) -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatHumanWB MouseHuman
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Immunogen
Other Immunogen Type. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HAP1, K562 and NIH/3T3 whole cell lysate. Mouse brain cortex lysates. IHC-P: Human breast tissue. Mouse and rat cerebrum tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
9G6 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-TLS/FUS (methyl ) antibody [9G6] (ab252830)All lanes : Anti-TLS/FUS (methyl ) antibody [9G6] (ab252830) at 1/500 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : TLS/FUS knockout HAP1 whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/10000 dilution
Predicted band size: 53 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: lanes 1-3: 10 seconds; lane 4: 37 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS (methyl ) antibody [9G6] (ab252830)](https://www.abcam.com/ps/products/252/ab252830/Images/ab252830-3-anti-none-antibody-9g6-immunohistochemistry-human-breast.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS (methyl ) antibody [9G6] (ab252830)Immunohistochemical analysis of paraffin-embedded human breast tissue labeling TLS/FUS with ab252830 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on human breast. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Western blot - Anti-TLS/FUS (methyl ) antibody [9G6] (ab252830)](https://www.abcam.com/ps/products/252/ab252830/Images/ab252830-397819-anti-none-antibody-9g6-western-blot-mouse-brain-cortex-tissue-lysate-40-ug.jpg)
Western blot - Anti-TLS/FUS (methyl ) antibody [9G6] (ab252830)Anti-TLS/FUS (methyl ) antibody [9G6] (ab252830) at 1/500 dilution + Mouse brain cortex tissue lysate at 40 µg
Secondary
Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/10000 dilution
Predicted band size: 53 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 46 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS (methyl ) antibody [9G6] (ab252830)](https://www.abcam.com/ps/products/252/ab252830/Images/ab252830-4-anti-none-antibody-9g6-immunohistochemistry-mouse-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS (methyl ) antibody [9G6] (ab252830)Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TLS/FUS with ab252830 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on mouse cerebrum. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS (methyl ) antibody [9G6] (ab252830)](https://www.abcam.com/ps/products/252/ab252830/Images/ab252830-5-anti-none-antibody-9g6-immunohistochemistry-rat-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS (methyl ) antibody [9G6] (ab252830)Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling TLS/FUS with ab252830 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on rat cerebrum. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
-
![Anti-TLS/FUS (methyl ) antibody [9G6] (ab252830)](https://www.abcam.com/ps/products/252/ab252830/Images/ab252830-6-benefits-of-recombinant-antibodies.png)
Anti-TLS/FUS (methyl ) antibody [9G6] (ab252830)
