Anti-TET1 antibody (ab272900)
Key features and details
- Rabbit polyclonal to TET1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TET1 antibody
See all TET1 primary antibodies -
Description
Rabbit polyclonal to TET1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human TET1 (internal sequence). The exact sequence is proprietary.
Database link: Q8NFU7 -
Positive control
- WB: HEK-293T, A431, HeLa, HepG2 cells. IHC-P: A549 xenograft, HeLa cells, Human normal prostate tissue. ICC/IF: HEK-293T cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TET1 antibody (ab272900)All lanes : Anti-TET1 antibody (ab272900) at 1/5000 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : DDDDK-human TET1 transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody (ab272900)Paraffin embedded A549 xenograft tissue stained for TET1 using ab272900 at 1/250 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-TET1 antibody (ab272900)4% paraformaldehyde-fixed Mock and transfected HEK-293T cells stained for TET1 (green) using ab272900 at 1/1000 dilution in ICC/IF analysis. Co-stained with Hoechest33342 (Blue).
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Western blot - Anti-TET1 antibody (ab272900)All lanes : Anti-TET1 antibody (ab272900) at 1/2000 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody (ab272900)Paraffin embedded HeLa xenograft tissue stained for TET1 using ab272900 at 1/1000 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
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Western blot - Anti-TET1 antibody (ab272900)All lanes : Anti-TET1 antibody (ab272900) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa nuclear extract lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody (ab272900)Paraffin embedded Human normal prostate tissue stained for TET1 using ab272900 at 1/1000 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.