Anti-TLR8 antibody [OTI4F3] (ab236521)
Key features and details
- Mouse monoclonal [OTI4F3] to TLR8
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-TLR8 antibody [OTI4F3]
See all TLR8 primary antibodies -
Description
Mouse monoclonal [OTI4F3] to TLR8 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human TLR8 aa 32-153. (NP_619542) produced in E.coli.
Database link: Q9NR97 -
Positive control
- IHC-P: Human spleen and spleen carcinoma tissue. WB: pCMV6-ENTRY TLR8 cDNA transfected HEK-293T cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI4F3 -
Isotype
IgG2a -
Research areas
Images
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Paraffin-embedded human spleen tissue stained for TLR8 using ab236521 at 1/2000 dilution in immunohistochemical analysis.
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All lanes : Anti-TLR8 antibody [OTI4F3] (ab236521) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY TLR8 cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 120 kDa
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Paraffin-embedded human spleen carcinoma tissue stained for TLR8 using ab236521 at 1/2000 dilution in immunohistochemical analysis.