Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TLR7 antibody [EPR2088(2)] (ab124928)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2088(2)] to TLR7
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-TLR7 antibody [EPR2088(2)]
    See all TLR7 primary antibodies

  • Description

    Rabbit monoclonal [EPR2088(2)] to TLR7

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human TLR7 aa 1000 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q9NYK1

  • Positive control

    • WB: Raji, Daudi and Ramos cell lysates. IHC-P: Human tonsil tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] (ab124928)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] (ab124928)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with unpurified ab124928 at 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-TLR7 antibody [EPR2088(2)] (ab124928)
    Western blot - Anti-TLR7 antibody [EPR2088(2)] (ab124928)
    All lanes : Anti-TLR7 antibody [EPR2088(2)] (ab124928) at 20 µg (unpurified)

    Lane 1 : Ramos cell lysate
    Lane 2 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

    Predicted band size: 121 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] (ab124928)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] (ab124928)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with purified ab124928 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-TLR7 antibody [EPR2088(2)] (ab124928)
    Western blot - Anti-TLR7 antibody [EPR2088(2)] (ab124928)
    All lanes : Anti-TLR7 antibody [EPR2088(2)] (ab124928) (purified)

    Lane 1 : Ramos cell lysate
    Lane 2 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 121 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-TLR7 antibody [EPR2088(2)] (ab124928)
    Western blot - Anti-TLR7 antibody [EPR2088(2)] (ab124928)
    All lanes : Anti-TLR7 antibody [EPR2088(2)] (ab124928) at 1/1000 dilution (unpurified)

    Lane 1 : Raji cell lysates
    Lane 2 : Ramos cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size: 121 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] (ab124928)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR7 antibody [EPR2088(2)] (ab124928)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling TLR7 with unpurified ab124928 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

  • OI-RD Scanning - Anti-TLR7 antibody [EPR2088(2)] (ab124928)
    OI-RD Scanning - Anti-TLR7 antibody [EPR2088(2)] (ab124928)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-TLR7 antibody [EPR2088(2)] (ab124928)
    Anti-TLR7 antibody [EPR2088(2)] (ab124928)

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