Anti-TLE4 antibody (ab140485)
Key features and details
- Rabbit polyclonal to TLE4
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-TLE4 antibody
See all TLE4 primary antibodies -
Description
Rabbit polyclonal to TLE4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Rabbit, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide corresponding to Mouse TLE4 aa 200-300 conjugated to keyhole limpet haemocyanin.
Database link: Q62441 -
Positive control
- This antibody gave a positive signal in the following tissue lysates: P7 Mouse Brain; P0 Mouse Brain; E16 Embryonic Mouse Brain; E16 Embryonic Rat Brain; E14 Embryonic Mouse Brain; E14 Embryonic Rat Brain; IHC-P: FFPE mouse brain E17 FFPE tissue sections.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab140485 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa). Target
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Relevance
TLE4 (Transducin-like enhancer protein 4) is a member of the Groucho family of transcriptional corepressors and inhibits the transcriptional activation mediated by Pax5 and by beta-catenin and TCF family members in Wnt signaling. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 21888 Mouse
- Entrez Gene: 25565 Rat
- SwissProt: Q62441 Mouse
- SwissProt: Q07141 Rat
- Unigene: 103638 Mouse
- Unigene: 53972 Rat
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Alternative names
- B lymphocyte gene 1 antibody
- E(sp1) homolog Drosophila antibody
- E(spI) antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE4 antibody (ab140485)
IHC image of TLE4 staining in mouse brain E17 formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab140485, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-TLE4 antibody (ab140485)All lanes : Anti-TLE4 antibody (ab140485) at 1 µg/ml
Lane 1 : P7 Mouse Brain Tissue Lysate Lab Lysate
Lane 2 : P0 Mouse Brain Mouse Tissue Lysate
Lane 3 : E16 Ms Embryo Brain Tissue Lysate
Lane 4 : E16 Rat Embryo Brain Tissue Lysate
Lane 5 : E14 Mouse Embryo Brain Tissue Lysate
Lane 6 : E14 Rat Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa (possible non-specific binding), 34 kDa (possible non-specific binding), 56 kDa (possible non-specific binding)
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab140485 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Protocols
Datasheets and documents
References (0)
ab140485 has not yet been referenced specifically in any publications.
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE4 antibody (ab140485)
IHC image of TLE4 staining in mouse brain E17 formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab140485, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-TLE4 antibody (ab140485)All lanes : Anti-TLE4 antibody (ab140485) at 1 µg/ml
Lane 1 : P7 Mouse Brain Tissue Lysate Lab Lysate
Lane 2 : P0 Mouse Brain Mouse Tissue Lysate
Lane 3 : E16 Ms Embryo Brain Tissue Lysate
Lane 4 : E16 Rat Embryo Brain Tissue Lysate
Lane 5 : E14 Mouse Embryo Brain Tissue Lysate
Lane 6 : E14 Rat Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa (possible non-specific binding), 34 kDa (possible non-specific binding), 56 kDa (possible non-specific binding)
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab140485 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
