Antibodies, Proteins, Kits and Reagents for Life Science

Anti-TLE3/ESG antibody [CL3573] (ab213596)

Key features and details

Mouse monoclonal [CL3573] to TLE3/ESG
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human
Isotype: IgG1

Product name

Anti-TLE3/ESG antibody [CL3573]
See all TLE3/ESG primary antibodies
Description

Mouse monoclonal [CL3573] to TLE3/ESG
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human TLE3/ESG aa 150-250.
Database link: Q04726

Run BLAST with Run BLAST with
Positive control
- WB: HEK293 cell lysate and HeLa nuclear lysate. IHC-P: Human cervix, tonsil, oral mucosa, small intestine, kidney and skeletal muscle tissues. ICC/IF: HeLa cells.
General notes

Previously labelled as TLE3.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

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Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

CL3573
Isotype

IgG1
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-TLE3/ESG antibody [CL3573] (ab213596)

PFA-fixed, Triton X-100 permeabilized HeLa cells stained for TLE3 (Green) using ab213596 at 4 μg/ml in ICC/IF.

Microtublues are stained red.
Western blot - Anti-TLE3/ESG antibody [CL3573] (ab213596)

Anti-TLE3/ESG antibody [CL3573] (ab213596) at 1/500 dilution + HEK293 cell lysate

Predicted band size: 83 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (ab213596)

Immunohistochemical analysis of Paraffin-embedded Human cervix tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (ab213596)

Immunohistochemical analysis of Paraffin-embedded Human tonsil tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (ab213596)

Immunohistochemical analysis of Paraffin-embedded Human oral mucosa tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (ab213596)

Immunohistochemical analysis of Paraffin-embedded Human small intestine tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (ab213596)

Immunohistochemical analysis of Paraffin-embedded Human kidney tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (ab213596)

Immunohistochemical analysis of Paraffin-embedded Human skeletal muscle tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.
Western blot - Anti-TLE3/ESG antibody [CL3573] (ab213596)

Anti-TLE3/ESG antibody [CL3573] (ab213596) at 1/500 dilution + HeLa nuclear lysate

Predicted band size: 83 kDa

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