Anti-TLE3/ESG antibody [CL3573] (ab213596)
![Anti-TLE3/ESG antibody [CL3573] (ab213596)](https://www.abcam.com/ps/products/213/ab213596/Images/ab213596-386730-anti-tle3-antibody-cl3573-hela-human.jpg "Anti-TLE3/ESG antibody [CL3573] (ab213596)")
Key features and details
- Mouse monoclonal [CL3573] to TLE3/ESG
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-TLE3/ESG antibody [CL3573]
See all TLE3/ESG primary antibodies -
Description
Mouse monoclonal [CL3573] to TLE3/ESG -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human TLE3/ESG aa 150-250.
Database link: Q04726 -
Positive control
- WB: HEK293 cell lysate and HeLa nuclear lysate. IHC-P: Human cervix, tonsil, oral mucosa, small intestine, kidney and skeletal muscle tissues. ICC/IF: HeLa cells.
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General notes
Previously labelled as TLE3.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL3573 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-TLE3/ESG antibody [CL3573] (ab213596)
PFA-fixed, Triton X-100 permeabilized HeLa cells stained for TLE3 (Green) using ab213596 at 4 μg/ml in ICC/IF.
Microtublues are stained red.
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![Western blot - Anti-TLE3/ESG antibody [CL3573] (ab213596)](https://www.abcam.com/ps/products/213/ab213596/Images/ab213596-269596-anti-tle3-esg-antibody-cl3573-western-blot.jpg)
Western blot - Anti-TLE3/ESG antibody [CL3573] (ab213596)Anti-TLE3/ESG antibody [CL3573] (ab213596) at 1/500 dilution + HEK293 cell lysate
Predicted band size: 83 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (ab213596)](https://www.abcam.com/ps/products/213/ab213596/Images/ab213596-269602-anti-tle3-esg-antibody-cl3573-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (ab213596)Immunohistochemical analysis of Paraffin-embedded Human cervix tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (ab213596)](https://www.abcam.com/ps/products/213/ab213596/Images/ab213596-269601-anti-tle3-esg-antibody-cl3573-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (ab213596)Immunohistochemical analysis of Paraffin-embedded Human tonsil tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (ab213596)](https://www.abcam.com/ps/products/213/ab213596/Images/ab213596-269600-anti-tle3-esg-antibody-cl3573-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (ab213596)Immunohistochemical analysis of Paraffin-embedded Human oral mucosa tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (ab213596)](https://www.abcam.com/ps/products/213/ab213596/Images/ab213596-269599-anti-tle3-esg-antibody-cl3573-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (ab213596)Immunohistochemical analysis of Paraffin-embedded Human small intestine tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (ab213596)](https://www.abcam.com/ps/products/213/ab213596/Images/ab213596-269598-anti-tle3-esg-antibody-cl3573-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (ab213596)Immunohistochemical analysis of Paraffin-embedded Human kidney tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (ab213596)](https://www.abcam.com/ps/products/213/ab213596/Images/ab213596-269597-anti-tle3-esg-antibody-cl3573-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (ab213596)Immunohistochemical analysis of Paraffin-embedded Human skeletal muscle tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.
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![Western blot - Anti-TLE3/ESG antibody [CL3573] (ab213596)](https://www.abcam.com/ps/products/213/ab213596/Images/ab213596-269595-anti-tle3-esg-antibody-cl3573-western-blot.jpg)
Western blot - Anti-TLE3/ESG antibody [CL3573] (ab213596)Anti-TLE3/ESG antibody [CL3573] (ab213596) at 1/500 dilution + HeLa nuclear lysate
Predicted band size: 83 kDa
