Anti-TIMP3 antibody - Carboxyterminal end (ab39185)
Key features and details
- Rabbit polyclonal to TIMP3 - Carboxyterminal end
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TIMP3 antibody - Carboxyterminal end
See all TIMP3 primary antibodies -
Description
Rabbit polyclonal to TIMP3 - Carboxyterminal end -
Host species
Rabbit -
Specificity
This antibody recognizes both the glycosylated and unglycosylated forms of TIMP3, and works against native or reduced TIMP3. It does not cross react with the other TIMP family members (TIMP1, TIMP2, TIMP4). -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide based on the carboxyterminal region of Human TIMP3.
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Positive control
- Human amd mouse TIMP3.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TIMP3 antibody - Carboxyterminal end (ab39185)Anti-TIMP3 antibody - Carboxyterminal end (ab39185) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 24 kDa
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
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Immunocytochemistry/ Immunofluorescence - Anti-TIMP3 antibody - Carboxyterminal end (ab39185)ICC/IF image of ab39185 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39185, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP3 antibody - Carboxyterminal end (ab39185)Ab39185 staining Human normal placenta. Staining is localized to the cytoplasm or excreted.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
