Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1053(N)(B)] to TIE1 (phospho Y1007) + TIE2 (phospho Y992)
  • Suitable for: WB, ICC/IF, Dot blot
  • Reacts with: Human

Overview

  • Product name

    Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)]

  • Description

    Rabbit monoclonal [EPR1053(N)(B)] to TIE1 (phospho Y1007) + TIE2 (phospho Y992)

  • Host species

    Rabbit

  • Specificity

    ab151704 only detects TIE1 phosphorylated at tyrosine 1007 and TIE2 phosphorylated at tyrosine 992.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    within Human TIE1 (phospho Y992). The exact sequence is proprietary.

  • Positive control

    • WB: HUVEC cell lysate treated with pervanadate. ICC/IF: HUVEC cells treated with pervanadate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
    Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
    All lanes : Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704) at 1/100000 dilution

    Lane 1 : HUVEC cell lysate treated with pervanadate
    Lane 2 : HUVEC cell lysate treated with pervanadate with TIE2 (phospho Y992) peptide
    Lane 3 : HUVEC cell lysate treated with pervanadate with TIE2 unmodified peptide
    Lane 4 : HUVEC cell lysate treated with pervanadate with TIE1 (phospho Y1007) peptide
    Lane 5 : HUVEC cell lysate treated with pervanadate with TIE1 unmodified peptide

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000000 dilution (HRP goat anti-rabbit IgG (H+L))

    Predicted band size: 125 kDa
    Observed band size: 125 kDa


    Exposure time: 5 seconds


    Blocking buffer: 5% BSA/TBST
    Dilution buffer: 5% BSA /TBST for primary antibody, 5% NFDM/TBST for secondary antibody

  • Immunocytochemistry/ Immunofluorescence - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
    Immunocytochemistry/ Immunofluorescence - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)

    Immunocytochemistry/Immunofluorescence analysis of HUVEC () cells labelling TIE2 + TIE1 (phospho Y992 + Y1007) with ab151704 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton-X. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291 anti-Tubulin (mouse mAb) at a dilution of 1/500 and ab150120 AlexaFluor®594 Goat anti-Mouse secondary at 1/1000. Nuclei were counterstained with DAPI (blue).

    Confocal image showing increased cytoplasmic staining after PER (Pervanadate, 1mM, 30min) treatment on HUVEC cells. The LP treatment decreased the PER induced cytoplasmic staining. 

  • Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
    Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
    All lanes : Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704) at 1/100000 dilution

    Lane 1 : Untreated HUVEC whole cell lysates
    Lane 2 : HUVEC treated with Pervanadate whole cell lysates
    Lane 3 : HUVEC treated with Pervanadate whole cell lysates, then the membrane was incubated with phosphatase.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 125 kDa
    Observed band size: 125 kDa


    Exposure time: 10 seconds


    Blocking/Diluting buffer 5% NFDM/TBST

  • Dot Blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
    Dot Blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)

    Dot blot analysis of TIE2 (pY992) phospho peptide (lane 1) and TIE2 non-phospho peptide (lane 2) labelling TIE2 (phospho Y992) with ab151704 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
    Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)

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