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Signal Transduction Protein Phosphorylation Tyrosine Kinases Receptor Tyrosine Kinases

Anti-EGFR (phospho Y1101) antibody [M199] (ab76195)

Price and availability

274 732 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-EGFR (phospho Y1101) antibody [M199] (ab76195)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [M199] to EGFR (phospho Y1101)
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-EGFR (phospho Y1101) antibody [M199]
    See all EGFR primary antibodies
  • Description

    Mouse monoclonal [M199] to EGFR (phospho Y1101)
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide corresponding to amino acid residues surrounding tyrosine 1101 of human EGFR conjugated to KLH

  • Positive control

    • ICC: A431 cells untreated or treated with EGF (100ng/ml) for 5 minutes; WB: A431 cell untreated or treated with EGF (100 ng/ml) for 5 minutes.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    M199
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • EGF
    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Cancer
    • Growth factors
    • EGF
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Tyrosine kinases
    • Receptor tyrosine kinases
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factor receptors
    • Cancer
    • Tumor biomarkers
    • Receptors

Images

  • Western blot - Anti-EGFR (phospho Y1101) antibody [M199] (ab76195)
    Western blot - Anti-EGFR (phospho Y1101) antibody [M199] (ab76195)
    All lanes : Anti-EGFR (phospho Y1101) antibody [M199] (ab76195) at 1/1000 dilution

    Lane 1 : A431 cells, untreated
    Lane 2 : A431 cells treated with EGF (100 ng/ml) for 5 minutes

    Predicted band size: 134 kDa
    Observed band size: 180 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1101) antibody [M199] (ab76195)
    Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1101) antibody [M199] (ab76195)
    ab76195 at 1/200 dilution staining EGFR in A431 cells, untreated (left) or treated with EGF (100ng/ml) for 5 min (right) by Immunoflurescence.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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