Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TID1 antibody [EPR12414] - BSA and Azide free (ab250291)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12414] to TID1 - BSA and Azide free
  • Suitable for: ICC, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TID1 antibody [EPR12414] - BSA and Azide free
    See all TID1 primary antibodies

  • Description

    Rabbit monoclonal [EPR12414] to TID1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250291 is the carrier-free version of ab181024. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250291 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TID1 antibody [EPR12414] - BSA and Azide free (ab250291)
    Western blot - Anti-TID1 antibody [EPR12414] - BSA and Azide free (ab250291)
    All lanes : Anti-TID1 antibody [EPR12414] (ab181024) at 1/5000 dilution

    Lane 1 : JAR cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 52 kDa



    This data was developed using ab181024, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-TID1 antibody [EPR12414] - BSA and Azide free (ab250291)
    Immunocytochemistry - Anti-TID1 antibody [EPR12414] - BSA and Azide free (ab250291)
    This data was developed using ab181024, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of acetone fixed HeLa cells labeling TID1 using ab181024 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution. Counterstain DAPI.
  • Immunoprecipitation - Anti-TID1 antibody [EPR12414] - BSA and Azide free (ab250291)
    Immunoprecipitation - Anti-TID1 antibody [EPR12414] - BSA and Azide free (ab250291)
    This data was developed using ab181024, the same antibody clone in a different buffer formulation.Lysate from Jurkat cells (Lane 1) and negative control (Lane 2) were immunoprecipitated with ab181024 at a 1/30 dilution. A specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.
  • Anti-TID1 antibody [EPR12414] - BSA and Azide free (ab250291)
    Anti-TID1 antibody [EPR12414] - BSA and Azide free (ab250291)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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