Anti-Thrombospondin 2 antibody (ab112543)
Key features and details
- Rabbit polyclonal to Thrombospondin 2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Thrombospondin 2 antibody
See all Thrombospondin 2 primary antibodies -
Description
Rabbit polyclonal to Thrombospondin 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to an internal sequence of Human Thrombospondin 2.
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Positive control
- WB: Hela, 22RV1, U-87MG, THP-1, PC-3, Caco-2 whole cell lysates. Mouse and rat brain tissue lysates. IHC-P: Human Placenta tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Thrombospondin 2 antibody (ab112543)All lanes : Anti-Thrombospondin 2 antibody (ab112543) at 0.5 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 22RV1 cell lysate
Lane 3 : U-87MG cell lysate
Lane 4 : THP-1 cell lysate
Lane 5 : PC-3 cell lysate
Lane 6 : Caco-2 cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG-HRP secondary antibody at 1/10000 dilution
Predicted band size: 130 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 2 antibody (ab112543)Thrombospondin 2/THBS2 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab112543 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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Western blot - Anti-Thrombospondin 2 antibody (ab112543)All lanes : Anti-Thrombospondin 2 antibody (ab112543) at 0.5 µg
Lane 1 : rat brain tissue lysates
Lane 2 : mouse brain tissue lysates
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG-HRP secondary antibody at 1/10000 dilution
Predicted band size: 130 kDa
