Anti-Thrombospondin 2 antibody (ab84469)
Key features and details
- Rabbit polyclonal to Thrombospondin 2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Thrombospondin 2 antibody
See all Thrombospondin 2 primary antibodies -
Description
Rabbit polyclonal to Thrombospondin 2 -
Host species
Rabbit -
Specificity
Reactivity in Rat is no longer guaranteed due to difficulty reproducing in-house experimental evidence, but will remain predicted, due to the immunogen showing 100% xr with Rat. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Horse, Chicken, Cow, Dog
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Immunogen
Synthetic peptide corresponding to Human Thrombospondin 2 aa 650-750 conjugated to keyhole limpet haemocyanin.
(Peptide available asab96113) -
Positive control
- This antibody gave a positive signal in both Human and Mouse spinal cord tissue lysate. This antibody gave a positive signal in ICC/IF in U2O2 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab84469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 129 kDa). ICC/IF Use a concentration of 10 µg/ml. Target
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Function
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin and type V collagen. -
Tissue specificity
High expression in invertebral disk tissue. -
Involvement in disease
Genetic variations in THBS2 may be a cause of susceptibility to intervertebral disc disease (IDD) [MIM:603932]; also known as lumbar disk herniation (LDH). IDD is one of the most common musculo-skeletal disorders and the predominant cause of low-back pain and unilateral leg pain. -
Sequence similarities
Belongs to the thrombospondin family.
Contains 3 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 1 TSP N-terminal (TSPN) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain. - Information by UniProt
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Database links
- Entrez Gene: 414837 Chicken
- Entrez Gene: 338092 Cow
- Entrez Gene: 7058 Human
- Entrez Gene: 21826 Mouse
- Entrez Gene: 292406 Rat
- Omim: 188061 Human
- SwissProt: P35440 Chicken
- SwissProt: Q95116 Cow
see all -
Alternative names
- THBS2 antibody
- Thrombospondin-2 antibody
- TSP2 antibody
- TSP2_HUMAN antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin 2 antibody (ab84469)
Ab84469 staining Thrombospondin 2 in U2O2 cells. The cells were fixed with 100% Methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab84469 at 10ugml then detected with an Alexa Fluor® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and tubulin was labelled using ab7291 Mouse monoclonal to alpha Tubulin together with ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594), at a 1/1000 dilution (shown in red).
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Western blot - Anti-Thrombospondin 2 antibody (ab84469)All lanes : Anti-Thrombospondin 2 antibody (ab84469) at 1 mg/ml
Lane 1 : Mouse spinal cord embryo E14
Lane 2 : Human skin
Lane 3 : Mouse skin
Lane 4 : A431 (Human epithelial carcinoma cell line)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 129 kDa
Additional bands at: 210 kDa (possible glycosylated form), 46 kDa (possible cleavage fragment), 58 kDa (possible non-specific binding)
Exposure time: 20 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with abX overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
ab84469 contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Protocols
Datasheets and documents
References (6)
ab84469 has been referenced in 6 publications.
- Pan W et al. TSP2 acts as a suppresser of cell invasion, migration and angiogenesis in medulloblastoma by inhibiting the Notch signaling pathway. Brain Res 1718:223-230 (2019). PubMed: 31063715
- Wu XG et al. Cancer-derived exosomal miR-221-3p promotes angiogenesis by targeting THBS2 in cervical squamous cell carcinoma. Angiogenesis N/A:N/A (2019). PubMed: 30993566
- Guo Y et al. Molecular characterization and functional analysis of Japanese flounder (Paralichthys olivaceus) thbs2 in response to lymphocystis disease virus. Fish Shellfish Immunol 93:183-190 (2019). PubMed: 31330254
- Du P et al. Downregulation of microRNA-1246 inhibits tumor growth and promotes apoptosis of cervical cancer cells by targeting thrombospondin-2. Oncol Lett 18:2491-2499 (2019). PubMed: 31404330
- Shen Y et al. MiR-106a aggravates sepsis-induced acute kidney injury by targeting THBS2 in mice model. Acta Cir Bras 34:e201900602 (2019). PubMed: 31432993
- Wang S et al. Macroglia-derived thrombospondin 2 regulates alterations of presynaptic proteins of retinal neurons following elevated hydrostatic pressure. PLoS One 12:e0185388 (2017). PubMed: 28953973
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin 2 antibody (ab84469)
Ab84469 staining Thrombospondin 2 in U2O2 cells. The cells were fixed with 100% Methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab84469 at 10ugml then detected with an Alexa Fluor® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and tubulin was labelled using ab7291 Mouse monoclonal to alpha Tubulin together with ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594), at a 1/1000 dilution (shown in red).
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Western blot - Anti-Thrombospondin 2 antibody (ab84469)All lanes : Anti-Thrombospondin 2 antibody (ab84469) at 1 mg/ml
Lane 1 : Mouse spinal cord embryo E14
Lane 2 : Human skin
Lane 3 : Mouse skin
Lane 4 : A431 (Human epithelial carcinoma cell line)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 129 kDa
Additional bands at: 210 kDa (possible glycosylated form), 46 kDa (possible cleavage fragment), 58 kDa (possible non-specific binding)
Exposure time: 20 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with abX overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
ab84469 contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
