Anti-THEA antibody [EPR18792] (ab180745)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18792] to THEA
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-THEA antibody [EPR18792]
See all THEA primary antibodies -
Description
Rabbit monoclonal [EPR18792] to THEA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal liver, heart and kidney tissue lysates; Mouse brown adipose, kidney, heart and spleen tissue lysates; NIH/3T3, Jurkat, LNCaP and HepG2 whole cell lysates.
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General notes
This product was previously labelled as ACOT11
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18792 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-THEA antibody [EPR18792] (ab180745) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 68 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 3 minutes; Lane 2/3: 15 seconds.
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All lanes : Anti-THEA antibody [EPR18792] (ab180745) at 1/2000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : LNCaP (human prostate cancer cell line) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 67,68 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 3 minutes; Lane 2/3: 15 seconds.
This antibody can recognize 2 isoforms in human, the predicted MW is 68 & 67 KDa.
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All lanes : Anti-THEA antibody [EPR18792] (ab180745) at 1/1000 dilution
Lane 1 : Mouse brown adipose lysate
Lane 2 : Mouse liver lysate
Lane 3 : Mouse kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 68 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
The expression profile observed is consistent with what has been described in the literature (PMID: 22427358 and PMID:11696000).
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All lanes : Anti-THEA antibody [EPR18792] (ab180745) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Mouse kidney lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 68 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/2: 1 minute; Lane 3: 10 seconds; Lane 4: 15 seconds.
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