Anti-TGM4 antibody (ab238563)
Key features and details
- Rabbit polyclonal to TGM4
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TGM4 antibody
See all TGM4 primary antibodies -
Description
Rabbit polyclonal to TGM4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
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Immunogen
Recombinant full length protein corresponding to Human TGM4 aa 1 to the C-terminus.
Database link: P49221 -
Positive control
- IHC-P: Human prostate tissue. ICC/IF: SH-SY5Y cells.
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General notes
Previously labelled as Transglutaminase 7.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGM4 antibody (ab238563)
Paraffin-embedded human prostate tissue stained for TGM4 using ab238563 at 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
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Immunocytochemistry/ Immunofluorescence - Anti-TGM4 antibody (ab238563)SH-SY5Y (human neuroblastoma cell line from bone marrow) cells stained for TGM4 (green) using ab238563 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L). The nuclear counter stain is DAPI.
The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.
