Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tetraspanin-33 antibody [EPR23121-146] - BSA and Azide free (ab272461)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23121-146] to Tetraspanin-33 - BSA and Azide free
  • Suitable for: Dot blot, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Tetraspanin-33 antibody [EPR23121-146] - BSA and Azide free
    See all Tetraspanin-33 primary antibodies

  • Description

    Rabbit monoclonal [EPR23121-146] to Tetraspanin-33 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Dot
    Recombinant fragment
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow cyt: Human PBMCs.

  • General notes

    ab272461 is the carrier-free version of ab238140. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23121-146

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-Tetraspanin-33 antibody [EPR23121-146] - BSA and Azide free (ab272461)
    Flow Cytometry - Anti-Tetraspanin-33 antibody [EPR23121-146] - BSA and Azide free (ab272461)

    Flow cytometric analysis of human peripheral blood mononuclear cells (PBMC) treated with 1ug/ml human CD40 ligand and 100U/ml human IL-4 for 72h labeling Tetraspanin-33 with ab238140 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG Isotype control (ab172730) (Left). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or ab238140 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238140).

     

  • Dot Blot - Anti-Tetraspanin-33 antibody [EPR23121-146] - BSA and Azide free (ab272461)
    Dot Blot - Anti-Tetraspanin-33 antibody [EPR23121-146] - BSA and Azide free (ab272461)

    Dot blot analysis of Tetraspanin-33 labeled with ab238140 at 1/1000 dilution.

    Lane 1: Recombinant tetraspanin-33 fragment containing the sequence at the immunogen region.
    Lane 2: BSA.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238140).

  • Anti-Tetraspanin-33 antibody [EPR23121-146] - BSA and Azide free (ab272461)
    Anti-Tetraspanin-33 antibody [EPR23121-146] - BSA and Azide free (ab272461)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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