Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Histone H3 (mono methyl K14) antibody [EPR17708-235] - BSA and Azide free (ab251366)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17708-235] to Histone H3 (mono methyl K14) - BSA and Azide free
Suitable for: PepArr, ICC, Flow Cyt, WB
Reacts with: Mouse, Human

Product name

Anti-Histone H3 (mono methyl K14) antibody [EPR17708-235] - BSA and Azide free
See all Histone H3 primary antibodies
Description

Rabbit monoclonal [EPR17708-235] to Histone H3 (mono methyl K14) - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: PepArr, ICC, Flow Cyt, WBmore details
Species reactivity

Reacts with: Mouse, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab251366 is the carrier-free version of ab202416. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251366 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Clonality

Monoclonal
Clone number

EPR17708-235
Isotype

IgG

Western blot - Anti-Histone H3 (mono methyl K14) antibody [EPR17708-235] - BSA and Azide free (ab251366)

All lanes : Anti-Histone H3 (mono methyl K14) antibody [EPR17708-235] (ab202416) at 1/1000 dilution

Lane 1 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 1 minute

This data was developed using ab202416, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Immunocytochemistry - Anti-Histone H3 (mono methyl K14) antibody [EPR17708-235] - BSA and Azide free (ab251366)

This data was developed using ab202416, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Histone H3 (mono methyl K14) with ab202416 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).Confocal image showing nuclear staining on HeLa cell line.The nuclear counter stain is DAPI (blue).Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:
-ve control 1: ab202416 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Flow Cytometry - Anti-Histone H3 (mono methyl K14) antibody [EPR17708-235] - BSA and Azide free (ab251366)

This data was developed using ab202416, the same antibody clone in a different buffer formulation.Flow cytometry analysis of HeLa cells labelling Histone H3 (mono methyl K14) (red) with purified ab202416 at dilution of 1/12 000. The secondary antibody used was Alexa Fluor^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody used was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
Peptide Array - Anti-Histone H3 (mono methyl K14) antibody [EPR17708-235] - BSA and Azide free (ab251366)

This data was developed using ab202416, the same antibody clone in a different buffer formulation.ab202416 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).

Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
Anti-Histone H3 (mono methyl K14) antibody [EPR17708-235] - BSA and Azide free (ab251366)

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