Anti-Tcl1 antibody (ab32813)
Key features and details
- Rabbit polyclonal to Tcl1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Tcl1 antibody
See all Tcl1 primary antibodies -
Description
Rabbit polyclonal to Tcl1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Tcl1.
Read Abcam's proprietary immunogen policy (Peptide available as ab38395.) -
Positive control
- Recombinant Human Tcl1 protein (ab87690) can be used as a positive control in WB. This antibody gave a positive signal in Ramos Whole Cell Lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32813 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).Target
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Function
Enhances the phosphorylation and activation of AKT1, AKT2 and AKT3. Promotes nuclear translocation of AKT1. Enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival. -
Tissue specificity
Restricted in the T-cell lineage to immature thymocytes and activated peripheral lymphocytes. Preferentially expressed early in T- and B-lymphocyte differentiation. -
Involvement in disease
Note=Chromosomal aberrations activating TCL1A are found in chronic T-cell leukemias (T-CLL). Translocation t(14;14)(q11;q32); translocation t(7;14)(q35;q32); inversion inv(14)(q11;q32) that involves the T-cell receptor alpha/delta loci. -
Sequence similarities
Belongs to the TCL1 family. -
Cellular localization
Cytoplasm. Nucleus. Microsome. Endoplasmic reticulum. Microsomal fraction. - Information by UniProt
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Database links
- Entrez Gene: 8115 Human
- Omim: 186960 Human
- SwissProt: P56279 Human
- Unigene: 2484 Human
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Alternative names
- Anti TCL1A antibody
- Lymphoma/leukemia, T-cell antibody
- Oncogene TCL-1 antibody
see all
Images
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Western blot - Anti-Tcl1 antibody (ab32813)Anti-Tcl1 antibody (ab32813) at 1 µg/ml + Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 13 kDa
Observed band size: 13 kDa
Datasheets and documents
References (0)
ab32813 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Tcl1 antibody (ab32813)Anti-Tcl1 antibody (ab32813) at 1 µg/ml + Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 13 kDa
Observed band size: 13 kDa
