Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TBX5 antibody [EPR23798-102] (ab259980)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23798-102] to TBX5
  • Suitable for: WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-TBX5 antibody [EPR23798-102]
    See all TBX5 primary antibodies

  • Description

    Rabbit monoclonal [EPR23798-102] to TBX5

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt (Intra),ICC,IHC-Fr,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse P0 heart, Mouse E18 heart, and Mouse E17 heart lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23798-102

  • Isotype

    IgG

Images

  • Western blot - (ab259980)
    Western blot - (ab259980)
    All lanes : Anti-TBX5 antibody [EPR23798-102] (ab259980) at 1/1000 dilution

    Lane 1 : Mouse P0 heart tissue lysate
    Lane 2 : Mouse P0 liver tissue lysate
    Lane 3 : Mouse E18 heart tissue lysate
    Lane 4 : Mouse E18 liver tissue lysate
    Lane 5 : Mouse E17 heart tissue lysate
    Lane 6 : Mouse E17 liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Observed band size: 57 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: mouse embryo/fetal liver (PIMID:16894625).

    Exposure time: 70 seconds

     

  • (ab259980)
    (ab259980)

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