Anti-TBX5 antibody [EPR23798-102] (ab259980)
![Anti-TBX5 antibody [EPR23798-102] (ab259980)](https://www.abcam.com/ps/products/259/ab259980/Images/ab259980-459581-anti-tbx5-antibody-epr23798102-western-blot-tissue.jpg "Anti-TBX5 antibody [EPR23798-102] (ab259980)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23798-102] to TBX5
- Suitable for: WB
- Reacts with: Mouse
Overview
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Product name
Anti-TBX5 antibody [EPR23798-102]
See all TBX5 primary antibodies -
Description
Rabbit monoclonal [EPR23798-102] to TBX5 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt (Intra),ICC,IHC-Fr,IHC-P or IP -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse P0 heart, Mouse E18 heart, and Mouse E17 heart lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23798-102 -
Isotype
IgG
Images
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Western blot - (ab259980)All lanes : Anti-TBX5 antibody [EPR23798-102] (ab259980) at 1/1000 dilution
Lane 1 : Mouse P0 heart tissue lysate
Lane 2 : Mouse P0 liver tissue lysate
Lane 3 : Mouse E18 heart tissue lysate
Lane 4 : Mouse E18 liver tissue lysate
Lane 5 : Mouse E17 heart tissue lysate
Lane 6 : Mouse E17 liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))
Observed band size: 57 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: mouse embryo/fetal liver (PIMID:16894625).
Exposure time: 70 seconds
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(ab259980)
