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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Tangles & Tau

Anti-Tau (phospho S235) antibody [EPR2452] (ab133253)

Price and availability

251 280 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-Tau (phospho S235) antibody [EPR2452] (ab133253)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2452] to Tau (phospho S235)
  • Suitable for: WB, Dot blot
  • Reacts with: Human

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Overview

  • Product name

    Anti-Tau (phospho S235) antibody [EPR2452]
    See all Tau primary antibodies
  • Description

    Rabbit monoclonal [EPR2452] to Tau (phospho S235)
  • Host species

    Rabbit
  • Specificity

    ab133253 reacts with Tau only when phosphorilated at Serine 235.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    within Human Tau aa 200-300. The exact sequence is proprietary.

  • Positive control

    • SH SY5Y cell lysates treated with Okadaic and Calyculin A.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2452
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Tangles & Tau
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • Tau
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker

Images

  • Western blot - Anti-Tau (phospho S235) antibody [EPR2452] (ab133253)
    Western blot - Anti-Tau (phospho S235) antibody [EPR2452] (ab133253)
    All lanes : Anti-Tau (phospho S235) antibody [EPR2452] (ab133253) at 1/1000 dilution

    Lane 1 : Human hippocampus lysate
    Lane 2 : Human hippocampus lysate - membrane incubated with phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody (HRP) at 1/2000 dilution

    Predicted band size: 78 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Dot Blot - Anti-Tau (phospho S235) antibody [EPR2452] (ab133253)
    Dot Blot - Anti-Tau (phospho S235) antibody [EPR2452] (ab133253)

    Dot blot analysis of Tau (pS235) peptide (Lane 1) and Tau non-phospho peptide (Lane 2) labelling Tau (pS235) with ab133253 at a dilution of 1/1000. ab97051 (Peroxidase-conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-Tau (phospho S235) antibody [EPR2452] (ab133253)
    Western blot - Anti-Tau (phospho S235) antibody [EPR2452] (ab133253)
    All lanes : Anti-Tau (phospho S235) antibody [EPR2452] (ab133253) at 1/1000 dilution

    Lane 1 : SH SY5Y cell lysates
    Lane 2 : SH SY5Y cell lysates treated with Okadaic acid and Calyculin A.

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 78 kDa



    Beta tubulin is shown as loading control in the bottom panel.
  • Anti-Tau (phospho S235) antibody [EPR2452] (ab133253)
    Anti-Tau (phospho S235) antibody [EPR2452] (ab133253)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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