Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tau antibody [PC1C6] (ab254150)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [PC1C6] to Tau
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Tau antibody [PC1C6]
    See all Tau primary antibodies

  • Description

    Mouse monoclonal [PC1C6] to Tau

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Full length native protein (purified) within Cow Tau. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P29172

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Human and mouse brain and hippocampus tissue lysate. Rat hippocampus tissue lysate. IHC-P: Human glioma and cerebrum tissue. Mouse cerebrum tissue. Rat hippocampus tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Tau antibody [PC1C6] (ab254150)
    Western blot - Anti-Tau antibody [PC1C6] (ab254150)
    All lanes : Anti-Tau antibody [PC1C6] (ab254150) at 0.77 µg/ml

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human hippocampus tissue lysate
    Lane 3 : Mouse brain tissue lysate
    Lane 4 : Mouse hippocampus tissue lysate
    Lane 5 : Rat hippocampus tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 46 kDa
    Observed band size: 37-70 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 24386422 and 3930508).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] (ab254150)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] (ab254150)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Tau with ab254150 at 1/5000 dilution (0.154µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human cerebrum. The section was incubated with ab254150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] (ab254150)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] (ab254150)

    Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling Tau with ab254150 at 1/5000 dilution (0.154µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human glioma. The section was incubated with ab254150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] (ab254150)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] (ab254150)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Tau with ab254150 at 1/10000 dilution (0.077µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on mouse cerebrum. The section was incubated with ab254150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] (ab254150)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau antibody [PC1C6] (ab254150)

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling Tau with ab254150 at 1/10000 dilution (0.077µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat hippocampus. The section was incubated with ab254150 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Tau antibody [PC1C6] (ab254150)
    Anti-Tau antibody [PC1C6] (ab254150)

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