Anti-TAOK1 antibody [EPR12908(2)] - BSA and Azide free (ab251247)
![Anti-TAOK1 antibody [EPR12908(2)] - BSA and Azide free (ab251247)](https://www.abcam.com/ps/products/251/ab251247/Images/ab251247-408136-anti-taok1-antibody-epr12908-2-bsa-and-azide-free-western-blot.jpg "Anti-TAOK1 antibody [EPR12908(2)] - BSA and Azide free (ab251247)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12908(2)] to TAOK1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-TAOK1 antibody [EPR12908(2)] - BSA and Azide free
See all TAOK1 primary antibodies -
Description
Rabbit monoclonal [EPR12908(2)] to TAOK1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251247 is the carrier-free version of ab197891. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251247 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12908(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TAOK1 antibody [EPR12908(2)] - BSA and Azide free (ab251247)All lanes : Anti-TAOK1 antibody [EPR12908(2)] (ab197891) at 1/1000 dilution
Lane 1 : Human seminoma tissue lysate
Lane 2 : Human testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 116 kDa
Exposure time: 1 minuteThis data was developed using ab197891, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-TAOK1 antibody [EPR12908(2)] - BSA and Azide free (ab251247)](https://www.abcam.com/ps/products/251/ab251247/Images/ab251247-405780-anti-taok1-antibody-epr12908-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-TAOK1 antibody [EPR12908(2)] - BSA and Azide free (ab251247)All lanes : Anti-TAOK1 antibody [EPR12908(2)] (ab197891) at 1/10000 dilution
Lane 1 : HeLa whole (Human epithelial cells from cervix adenocarcinoma) cell lysate
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 116 kDa
Exposure time: 1 minuteThis data was developed using ab197891, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-TAOK1 antibody [EPR12908(2)] - BSA and Azide free (ab251247)](https://www.abcam.com/ps/products/251/ab251247/Images/ab251247-403167-anti-taok1-antibody-epr12908-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-TAOK1 antibody [EPR12908(2)] - BSA and Azide free (ab251247)All lanes : Anti-TAOK1 antibody [EPR12908(2)] (ab197891) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat heart lysate
Lane 3 : Rat spleen lysate
Lane 4 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 116 kDa
Exposure time: 1 minuteThis data was developed using ab197891, the same antibody clone in a different buffer formulation.
-
![Western blot - Anti-TAOK1 antibody [EPR12908(2)] - BSA and Azide free (ab251247)](https://www.abcam.com/ps/products/251/ab251247/Images/ab251247-399249-anti-taok1-antibody-epr12908-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-TAOK1 antibody [EPR12908(2)] - BSA and Azide free (ab251247)This data was developed using ab197891, the same antibody clone in a different buffer formulation.
Immunoprecipitation from Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extract using ab197891 at 1/100 dilution for capture (IP +) or Rabbit monoclonal IgG ab172730 (IP -).
For WB ab197891 was used at 1/5000 dilution in 5% NFDM/TBST buffer. Input is Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extract. Secondary antibody was anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
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![Anti-TAOK1 antibody [EPR12908(2)] - BSA and Azide free (ab251247)](https://www.abcam.com/ps/products/251/ab251247/Images/ab251247-5-benefits-of-recombinant-antibodies.png)
Anti-TAOK1 antibody [EPR12908(2)] - BSA and Azide free (ab251247)