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Anti-Talin 1 antibody [97H6] - BSA and Azide free (ab256125)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [97H6] to Talin 1 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Talin 1 antibody [97H6] - BSA and Azide free
    See all Talin 1 primary antibodies

  • Description

    Mouse monoclonal [97H6] to Talin 1 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HUVEC, HeLa, HepG2, NIH/3T3, MEF and C6 whole cell lysate. IP: NIH/3T3 whole cell lysate. IHC-P: Mouse spleen tissue.

  • General notes

    ab256125 is the carrier-free version of ab108480. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256125 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Talin 1 antibody [97H6] - BSA and Azide free (ab256125)
    Western blot - Anti-Talin 1 antibody [97H6] - BSA and Azide free (ab256125)
    All lanes : Anti-Talin 1 antibody [97H6] (ab108480) at 0.481 µg/ml

    Lane 1 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 4 : C6 (rat glial tumor glial cell), whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 6 : MEF (mouse embryonic fibroblast (immortalized)), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : eroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 269 kDa


    Exposure time: 3 seconds


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108480).

    The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID:22306379 and 25751747).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Talin 1 antibody [97H6] - BSA and Azide free (ab256125)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Talin 1 antibody [97H6] - BSA and Azide free (ab256125)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Talin 1 with ab108480 at 1/5000 dilution (0.241µg/ml) followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on mouse spleen. The section was incubated with ab108480 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108480).

  • Immunoprecipitation - Anti-Talin 1 antibody [97H6] - BSA and Azide free (ab256125)
    Immunoprecipitation - Anti-Talin 1 antibody [97H6] - BSA and Azide free (ab256125)

    Talin 1 was immunoprecipitated from NIH/3T3 (mouse embryonic fibroblast), whole cell lysate with ab108480 at 1/30 dilution (2µg in 0.35mg lysates) (16.033µg/ml). Western blot was performed on the immunoprecipitate using ab108480 at 1/1000 dilution (0.481µg/ml). Mouse IgG for IP (HRP) (ab131368) was used at 1/1000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10µg.

    Lane 2: ab108480 IP in NIH/3T3 whole cell lysate.

    Lane 3: Mouse monoclonal IgG1 (ab18443) instead of ab108480 in NIH/3T3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108480).

  • Anti-Talin 1 antibody [97H6] - BSA and Azide free (ab256125)
    Anti-Talin 1 antibody [97H6] - BSA and Azide free (ab256125)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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