Anti-Tal1 antibody (ab155195)
Key features and details
- Rabbit polyclonal to Tal1
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Tal1 antibody
See all Tal1 primary antibodies -
Description
Rabbit polyclonal to Tal1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide, corresponding to a region within C terminal amino acids 252-331 of Human Tal1 (Uniprot ID: P17542).
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Positive control
- WB: H1299 cell lysate; IHC-P: Mouse spleen tissue, A549 xenograft; IP: HEK-293T cell lysate.
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General notes
Keep as a concentrated solution.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-Tal1 antibody (ab155195) at 1/1000 dilution + H1299 (human non-small cell lung carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 34 kDa10% SDS PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tal1 antibody - C-terminal (ab155195)
Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue staining Tal1 with ab155195 at 1/100 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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TAL1 antibody immunoprecipitates TAL1 protein in IP experiments.
IP Sample: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate.
A: HEK-293T whole cell lysate. 40 μg.
B: Control with 2 μg of preimmune rabbit IgG.
C: Immunoprecipitate of TAL1 protein by 2 μg of ab155195.
12% SDS-PAGE
The immunoprecipitated TAL1 protein was detected by ab155195 at 1/1000 dilution, followed by anti-rabbit IgG antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tal1 antibody (ab155195)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue staining Tal1 at cytosol and nucleus with ab155195 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.