Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free (ab251558)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20331] to AP2 gamma/TFAP2C - BSA and Azide free
  • Suitable for: Flow Cyt, ICC, IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free
    See all AP2 gamma/TFAP2C primary antibodies

  • Description

    Rabbit monoclonal [EPR20331] to AP2 gamma/TFAP2C - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC, IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251558 is the carrier-free version of ab218107 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251558 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as AP2 gamma

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free (ab251558)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free (ab251558)

    This data was developed using ab218107, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling AP2 gamma/TFAP2C with ab218107 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human normal breast is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free (ab251558)
    Immunocytochemistry - Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free (ab251558)

    This data was developed using ab218107, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-BR-3 (Human mammary gland adenocarcinoma cell line) cells labeling AP2 gamma/TFAP2C with ab218107 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on SK-BR-3 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
  • Western blot - Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free (ab251558)
    Western blot - Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free (ab251558)
    Lanes 1-2 : Anti-AP2 gamma/TFAP2C antibody [EPR20331] (ab218107) at 1/1000 dilution
    Lanes 3-4 : Anti-AP2 gamma/TFAP2C antibody [EPR20331] (ab218107) at 1/2000 dilution

    Lane 1 : SK-BR-3 (Human mammary gland adenocarcinoma cell line) whole cell lysate
    Lane 2 : MDA-MB-435S (Human ductal carcinoma cell line) whole cell lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 4 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 49 kDa
    Observed band size: 49 kDa



    This data was developed using ab218107, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 30 seconds; Lane 3: 10 seconds; Lane 4: 3 minutes.

  • Flow Cytometry - Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free (ab251558)
    Flow Cytometry - Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free (ab251558)

    This data was developed using ab218107, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed SK-BR-3 (Human mammary gland adenocarcinoma cell line) cells labeling AP2 gamma/TFAP2C with ab218107 at 1/50 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
  • Immunoprecipitation - Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free (ab251558)
    Immunoprecipitation - Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free (ab251558)

    This data was developed using ab218107, the same antibody clone in a different buffer formulation.

    AP2 gamma/TFAP2C was immunoprecipitated from 0.35 mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab218107 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab218107 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: MCF7 whole cell lysate, 10 µg (Input).

    Lane 2: ab218107 IP in MCF7 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab2018107 in MCF7 whole cell lysate.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free (ab251558)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free (ab251558)
    This data was developed using ab218107, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling AP2 gamma/TFAP2C with ab218107 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on human placenta is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free (ab251558)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free (ab251558)
    This data was developed using ab218107, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling AP2 gamma/TFAP2C with ab218107 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on poorly differentiated human breast cancer is observed. The data is consistent with the literature (PMID: 18825690). Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free (ab251558)
    Immunocytochemistry - Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free (ab251558)
    This data was developed using ab218107, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling AP2 gamma/TFAP2C with ab218107 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on MCF7 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free (ab251558)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free (ab251558)
    This data was developed using ab218107, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human seminoma tissue labeling AP2 gamma/TFAP2C with ab218107 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nucleus staining on human seminoma is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free (ab251558)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free (ab251558)
    This data was developed using ab218107, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling AP2 gamma/TFAP2C with ab218107 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Almost all tumor cells show negative staining on moderately differentiated human breast cancer. The data is consistent with the literature (PMID: 18825690). Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free (ab251558)
    Anti-AP2 gamma/TFAP2C antibody [EPR20331] - BSA and Azide free (ab251558)

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