Anti-TAF6 antibody [585D4a] (ab51026)
Key features and details
- Mouse monoclonal [585D4a] to TAF6
- Suitable for: WB, IP, ICC
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-TAF6 antibody [585D4a]
See all TAF6 primary antibodies -
Description
Mouse monoclonal [585D4a] to TAF6 -
Host species
Mouse -
Tested applications
Suitable for: WB, IP, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment
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Positive control
- HEK293 whole cell lysate. HeLa cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified using protein G chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 micrometer membrane. -
Clonality
Monoclonal -
Clone number
585D4a -
Isotype
IgG1 -
Research areas
Images
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Anti-TAF6 antibody [585D4a] (ab51026) at 1/50 dilution + HEK293 whole cell lysate at 25 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 73 kDa
Observed band size: 73 kDa
Additional bands at: 105 kDa. We are unsure as to the identity of these extra bands.
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ab51026 was used at 5µg/500µg of RIPA lysate of HeLa cells. Western blot was labelled with ab51026 at 1/50 dilution followed by Mouse IgG antibody at 1/2500 dilution and developed using the ECL technique.
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HeLa cells labelled with ab51026 at 1/5 dilution. Alexa Fluor 488 Goat anti-Mouse IgG was used as secondary antibody at 1/200 dilution.