Anti-MICA + MICB antibody [6D4] (ab54413)
![Anti-MICA + MICB antibody [6D4] (ab54413)](https://www.abcam.com/ps/products/54/ab54413/Images/ab54413-456746-anti-mica--micb-antibody-6d4-flow-cytometry-jurkat.jpg "Anti-MICA + MICB antibody [6D4] (ab54413)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [6D4] to MICA + MICB
- Suitable for: Flow Cyt
- Reacts with: Human
Overview
-
Product name
Anti-MICA + MICB antibody [6D4]
See all MICA + MICB primary antibodies -
Description
Mouse monoclonal [6D4] to MICA + MICB -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: IHC-P or WB -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Flow Cyt: HeLa cells.
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
Clone 6D4 is reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells -
Clonality
Monoclonal -
Clone number
6D4 -
Isotype
IgG2a -
Research areas
Images
-
Flow Cytometry - Anti-MICA + MICB antibody [6D4] (ab54413)
Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, Left) / HeLa (Human cervix adenocarcinoma epithelial cell, Right) cells labelling MICA + MICB with ab54413 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
Negative control: Jurkat.
Gated on viable cells.
-
![Anti-MICA + MICB antibody [6D4] (ab54413)](https://www.abcam.com/ps/products/54/ab54413/Images/ab54413-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-MICA + MICB antibody [6D4] (ab54413)
