Antibodies, Proteins, Kits and Reagents for Life Science

Anti-PCGF6 antibody [EPR19450] - BSA and Azide free (ab251294)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19450] to PCGF6 - BSA and Azide free
Suitable for: WB, IP, ICC
Reacts with: Mouse, Rat

Product name

Anti-PCGF6 antibody [EPR19450] - BSA and Azide free
See all PCGF6 primary antibodies
Description

Rabbit monoclonal [EPR19450] to PCGF6 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, IP, ICCmore details
Species reactivity

Reacts with: Mouse, Rat
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
General notes
ab251294 is the carrier-free version of ab200038 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab251294 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.

This product was previously labelled as RING6a
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Clonality

Monoclonal
Clone number

EPR19450
Isotype

IgG
Research areas

Western blot - Anti-PCGF6 antibody [EPR19450] - BSA and Azide free (ab251294)

All lanes : Anti-PCGF6 antibody [EPR19450] (ab200038) at 1/1000 dilution

Lane 1 : Mouse testis lysate
Lane 2 : Mouse thymus lysate
Lane 3 : Mouse ovary lysate
Lane 4 : Mouse embryo lysate
Lane 5 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 40 kDa
Observed band size: 42 kDa
why is the actual band size different from the predicted?

This data was developed using ab200038, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1-4: 3 minutes; Lane 5: 10 seconds.
Western blot - Anti-PCGF6 antibody [EPR19450] - BSA and Azide free (ab251294)

All lanes : Anti-PCGF6 antibody [EPR19450] (ab200038) at 1/1000 dilution

Lane 1 : Rat testis lysate
Lane 2 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 3 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 40 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?

This data was developed using ab200038, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times: Lanes 1-2 : 3 minutes; Lanes 3-4: 30 seconds.
Immunocytochemistry - Anti-PCGF6 antibody [EPR19450] - BSA and Azide free (ab251294)

This data was developed using ab200038, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized F9 (Mouse embryonic testicular cancer cell line) cells labeling PCGF6 with ab200038 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on F9 cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red). The negative controls are as follows:- -ve control 1: ab200038 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution. -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunoprecipitation - Anti-PCGF6 antibody [EPR19450] - BSA and Azide free (ab251294)

This data was developed using ab200038&

44; the same antibody clone in a different buffer formulation.
>ab200038 Immunoprecipitating PCGF6 in F9 (mouse embryonal carcinoma) whole cell lysate. 350µg of cell lysate was incubated with primary antibody (2 µg/ml). For western blotting, ab200038 (1/1000) was used to confirm successful immunoprecipation. For detection VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000.
Lane 1: (Input) F9 (mouse embryonal carcinoma) whole cell lysate 10µg

Lane 2: (+) F9 whole cell lysate

Lane 3: (-) Rabbit monoclonal IgG (ab172730) instead of ab200038 in F9 whole cell lysate
Anti-PCGF6 antibody [EPR19450] - BSA and Azide free (ab251294)

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