Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SynGAP antibody [EPR2883Y] - BSA and Azide free (ab247491)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2883Y] to SynGAP - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SynGAP antibody [EPR2883Y] - BSA and Azide free
    See all SynGAP primary antibodies

  • Description

    Rabbit monoclonal [EPR2883Y] to SynGAP - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247491 is the carrier-free version of ab77235. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247491 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SynGAP antibody [EPR2883Y] - BSA and Azide free (ab247491)
    Western blot - Anti-SynGAP antibody [EPR2883Y] - BSA and Azide free (ab247491)
    All lanes : Anti-SynGAP antibody [EPR2883Y] (ab77235) at 1/1000 dilution

    Lane 1 : Hippocampus (Rat) Tissue Lysate
    Lane 2 : Cortex (Rat) Tissue Lysate
    Lane 3 : Hippocampus (Mouse) Tissue Lysate
    Lane 4 : Cortex (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 147 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 minutes


    This data was developed using ab77235, the same antibody clone in a different buffer formulation.

    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab77235 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    The doublet observed in the Western blot image is consistent with the literature (PMID: 9581761).

  • Western blot - Anti-SynGAP antibody [EPR2883Y] - BSA and Azide free (ab247491)
    Western blot - Anti-SynGAP antibody [EPR2883Y] - BSA and Azide free (ab247491)
    Anti-SynGAP antibody [EPR2883Y] (ab77235) at 1/1000 dilution + fetal brain lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 147 kDa
    Observed band size: 147 kDa
    Additional bands at: 50 kDa, 55 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab77235, the same antibody clone in a different buffer formulation.

     

  • Anti-SynGAP antibody [EPR2883Y] - BSA and Azide free (ab247491)
    Anti-SynGAP antibody [EPR2883Y] - BSA and Azide free (ab247491)

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