Anti-Synaptotagmin antibody (ab51164)
Key features and details
- Rabbit polyclonal to Synaptotagmin
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Synaptotagmin antibody
See all Synaptotagmin primary antibodies -
Description
Rabbit polyclonal to Synaptotagmin -
Host species
Rabbit -
Specificity
ab51164 detects endogenous levels of total Synaptotagmin. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthesized non-phosphopeptide derived from human Synaptotagmin around the phosphorylation site of serine 309.
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Positive control
- 293 cell extract treated with PMA (125ng/ml, 30min) and human breast carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptotagmin antibody (ab51164)Immunohistochemical analysis of human breast carcinoma tissue (paraffin section) using ab51164 (1/50 dilution); with and without peptide.
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Western blot - Anti-Synaptotagmin antibody (ab51164)All lanes : Anti-Synaptotagmin antibody (ab51164) at 1/500 dilution
Lane 1 : 293 cell extract treated with
PMA (125ng/ml, 30min); without peptide
Lane 2 : 293 cell extract treated with
PMA (125ng/ml, 30min); with peptide
Predicted band size: 48 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
