Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23500-32] to SV2A - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr, Flow Cyt, IP, Dot blot, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SV2A antibody [EPR23500-32] - BSA and Azide free
    See all SV2A primary antibodies

  • Description

    Rabbit monoclonal [EPR23500-32] to SV2A - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IHC-Fr
    Mouse
    IHC-P
    Human
    IP
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain and hippocampus tissue lysates; Mouse brain and hippocampus tissue lysates; Rat brain and hippocampus tissue lysates. IHC-P: Human cerebrum and pancreas tissue; Mouse cerebrum and pancreas tissue; Rat cerebrum and pancreas tissue. IHC-Fr: Mouse pancreas and cerebrum tissue; Rat pancreas and cerebrum tissue. ICC/IF: Mouse primary neuron cells. Flow Cyt: Neuro-2a cells. IP: Mouse brain tissue lysate.

  • General notes

    ab273513 is the carrier-free version of ab254351. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)
    Dot Blot - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)

    This data was developed using the same antibody clone in a different buffer formulation (ab254351).

    Concentration of ab254351: 1/1000 dilution

    Secondary ab: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/100000 dilution 

    Blocking/diluting buffer and concentration: 5% NFDM/TBST

    Lane 1: SV2A immunogen peptide

    Lane 2: SV2C corresponding region of SV2A immunogen peptide

    Lane 3: SV2A non-immunogen peptide

    Exposure time: 3 minutes 

  • Immunohistochemistry (Frozen sections) - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)
    Immunohistochemistry (Frozen sections) - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pancreas tissue labeling SV2A with ab254351 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse pancreatic islet is observed. Insulin is counter stained using ab6995 Anti-Insulin mouse monoclonal antibody. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254351).

  • Immunohistochemistry (Frozen sections) - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)
    Immunohistochemistry (Frozen sections) - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat pancreas tissue labeling SV2A with ab254351 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat pancreatic islet is observed. Insulin is counter stained using ab6995 Anti-Insulin mouse monoclonal antibody. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254351).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling SV2A with ab254351 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in mouse cerebrum (PMID:16306227). The section was incubated with ab254351 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254351).

  • Immunocytochemistry/ Immunofluorescence - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)
    Immunocytochemistry/ Immunofluorescence - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cell cells labelling SV2A with ab254351 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254351).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling SV2A with ab254351 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in rat cerebrum (PMID:16306227). The section was incubated with ab254351 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254351).

  • Flow Cytometry - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)
    Flow Cytometry - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling SV2A with ab254351 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254351).

  • Immunoprecipitation - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)
    Immunoprecipitation - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)

    SV2A was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with ab254351 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using 254351 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10 ug

    Lane 2: 254351 IP in Mouse brain tissue lysate

    Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab254551 in Mouse brain tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3.25 seconds.

    Samples are non-boiled as boiling may cause protein aggregates.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254351).

  • Immunohistochemistry (Frozen sections) - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)
    Immunohistochemistry (Frozen sections) - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum tissue labeling SV2A with ab254351 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254351).

  • Immunohistochemistry (Frozen sections) - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)
    Immunohistochemistry (Frozen sections) - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling SV2A with ab254351 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254351).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling SV2A with ab254351 at 1/1000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in human cerebrum (PMID:16306227). The section was incubated with ab254351 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254351).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)

    Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling SV2A with ab254351 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in rat pancreatic islet (PMID:16306227). The section was incubated with ab254351 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254351).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)

    Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling SV2A with ab254351 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in mouse pancreatic islet (PMID:16306227). The section was incubated with ab254351 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254351).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling SV2A with ab254351 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in human pancreatic islet (PMID:16306227). The section was incubated with ab254351 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254351).

  • Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)
    Anti-SV2A antibody [EPR23500-32] - BSA and Azide free (ab273513)

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