Anti-Survivin DEx3 antibody (ab3731)
Key features and details
- Rabbit polyclonal to Survivin DEx3
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Survivin DEx3 antibody -
Description
Rabbit polyclonal to Survivin DEx3 -
Host species
Rabbit -
Specificity
This antibody has been tested in Western blotting against equal loadings of purified wild type survivin, 2B and DEx3. When used at 1/250, it detects a single clean (but weak) band of the appropriate size for DEx3, but no band is detected with wild type survivin or 2B. We have not succeeded in detecting endogenous Survivin DEx3 using this antibody (Survivin DEx3 is expressed at very low levels). This antibody has been used successfully however, to detect overexpressed Survivin DEx3 in whole cell extracts. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic peptide corresponding to Human Survivin DEx3 aa 50-150.
(Peptide available asab13763) -
Positive control
- This antibody gave a positive result in HeLa cell line in ICC/IF
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General notes
This antibody should be aliquoted to reduce freeze-thaw cycles as it loses performance quickly after multiple freeze-thaws.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanApplication Abreviews Notes ICC/IF Use a concentration of 5 µg/ml.Notes ICC/IF
Use a concentration of 5 µg/ml.Target
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Relevance
Survivin is expressed in G2/M phase in a cycle regulated manner. At the beginning of mitosis, survivin associates with mitotic spindle microtubules, this is essential for survivin's anti-apoptosis function. Survivin may counteract a default induction of apoptosis in G2 / M phase. Survivin over-expression in cancer may overcome this apoptotic checkpoint. Survivin DEx3 (lacking exon 3) and survivin-2B (retaining a part of intron 2 as a cryptic exon) are isoforms of survivin that behave differently to survivin. Skipping of exon 3 in survivin-Ex3 results in a modification of the BIR domain, followed by an additional frame shift in exon 4. In consequence, the open reading frame of Survivin DEx3 ends in the 3' untranslated region of survivin at position 581 and generates a novel 63 amino acids-long COOH-terminal tail in survivin-Ex3 coded by 87 nucleotides of exon 4 and 103 nucleotides of the original 3' untranslated region. See Mahotka et al. 1999 for more details. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 332 Human
- Omim: 603352 Human
- SwissProt: O15392 Human
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Alternative names
- API4 antibody
- Apoptosis Inhibitor 4 antibody
- BIRC5 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Survivin DEx3 antibody (ab3731)ICC/IF image of ab3731 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3731, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (6)
ab3731 has been referenced in 6 publications.
- Waligórska-Stachura J et al. Survivin DEx3 as a biomarker of thyroid cancers: A study at the mRNA and protein level. Oncol Lett 13:2437-2441 (2017). IHC-P ; Human . PubMed: 28454416
- Waligorska-Stachura J et al. Decreased expression of survivin 2B in human pituitary adenomas. A preliminary study. Folia Histochem Cytobiol 55:21-25 (2017). PubMed: 28509313
- Doucette T et al. Survivin transcript variant 2 drives angiogenesis and malignant progression in proneural gliomas. Neuro Oncol N/A:N/A (2014). Mouse . PubMed: 24676140
- Végran F et al. Association of p53 gene alterations with the expression of antiapoptotic survivin splice variants in breast cancer. Oncogene 26:290-7 (2007). PubMed: 16847456
- Noton EA et al. Molecular analysis of survivin isoforms: evidence that alternatively spliced variants do not play a role in mitosis. J Biol Chem 281:1286-95 (2006). PubMed: 16291752
- Caldas H et al. Survivin splice variants regulate the balance between proliferation and cell death. Oncogene 24:1994-2007 (2005). PubMed: 15688031
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Survivin DEx3 antibody (ab3731)ICC/IF image of ab3731 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3731, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.