Anti-Oct6 antibody [EPR24057-94] (ab259952)
![Anti-Oct6 antibody [EPR24057-94] (ab259952)](https://www.abcam.com/ps/products/259/ab259952/Images/ab259952-459577-anti-oct6-antibody-epr2405794-western-blot-tissue.jpg "Anti-Oct6 antibody [EPR24057-94] (ab259952)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24057-94] to Oct6
- Suitable for: ICC, WB, IHC-P, IHC-Fr, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Oct6 antibody [EPR24057-94]
See all Oct6 primary antibodies -
Description
Rabbit monoclonal [EPR24057-94] to Oct6 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, IHC-P, IHC-Fr, Flow Cytmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat P0 brain, Rat P7 hippocampus, Mouse P1 brain, F9, NCCIT lysates. IHC-P: Human skin, Mouse cerebrum and Rat skin tissues. IHC-Fr: Mouse E14.5 embryonic, Rat E14.5 embryonic tissues. ICC: rat primary neuron, mouse primary neuron cellss. Flow Cyt: Mouse primary neuron, Rat primary neuron cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24057-94 -
Isotype
IgG
Images
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Western blot - Anti-Oct6 antibody [EPR24057-94] (ab259952)All lanes : Anti-Oct6 antibody [EPR24057-94] (ab259952) at 1/1000 dilution
Lane 1 : Rat P0 brain tissue lysate
Lane 2 : Rat P7 hippocampus tissue lysate
Lane 3 : Rat lung tissue lysate
Lane 4 : Mouse P1 brain tissue lysate
Lane 5 : Mouse lung tissue lysate
Lane 6 : F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate
Lane 7 : NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 45 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: lung (PMID: 1979677).
Exposure time: 3 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259952)Immunohistochemical analysis of paraffin-embedded Human skin tissue labelling Oct6 with ab259952 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on epithelial cells of human skin. The section was incubated with ab259952 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259952)Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling Oct6 with ab259952 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse cerebrum. The section was incubated with ab259952 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259952)Immunohistochemical analysis of paraffin-embedded Rat skin tissue labelling Oct6 with ab259952 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on epithelial cells of rat skin. The section was incubated with ab259952 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab259952)Immunohistochemical analysis of paraffin-embedded Rat lung tissue labelling Oct6 with ab259952 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in rat lung. The section was incubated with ab259952 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemistry (Frozen sections) - (ab259952)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse E14.5 embryonic tissue labeling Oct6 with ab259952 at 1/100 (5.22 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Nuclear staining on mouse E14.5 embryonic brain is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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Immunohistochemistry (Frozen sections) - (ab259952)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat E14.5 embryonic tissue labeling Oct6 with ab259952 at 1/100 (5.22 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Nuclear staining on rat E14.5 embryonic brain is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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Immunocytochemistry - (ab259952)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling Oct6 with ab259952 at 1/50 (10.44 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing nuclear staining in rat primary neuron.Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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Immunocytochemistry - (ab259952)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Oct6 with ab259952 at 1/50 (10.44 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing nuclear staining in mouse primary neuron.Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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Flow Cytometry - (ab259952)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells cells labelling Oct6 with ab259952 at 1/50 dilution (1ug)/(Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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Flow Cytometry - (ab259952)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neuron cells cells labelling Oct6 with ab259952 at 1/50 dilution (1ug)/(Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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(ab259952)
