Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-Sumo 1 antibody [EP298] - BSA and Azide free
See all Sumo 1 primary antibodies
Description

Rabbit monoclonal [EP298] to Sumo 1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab248491 is the carrier-free version of ab133352. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab248491 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP298
Isotype

IgG
Research areas

Western blot - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)

This data was developed using ab133352, the same antibody clone in a different buffer formulation.

Lane 1: Wild-type HAP1 cell lysate (20 µg)

Lane 2: Sumo 1 knockout HAP1 cell lysate (20 µg)

Lane 3: HeLa cell lysate (20 µg)

Lane 4: MCF-7 cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab133352 observed at 16 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab133352 was shown to recognize Sumo 1 when Sumo 1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Sumo 1 knockout samples were subjected to SDS-PAGE. ab133352 and ab8245 (loading control to GAPDH) were diluted 1/000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)

This data was developed using ab133352, the same antibody clone in a different buffer formulation.

Immunohistochemical staining of paraffin-embedded human thyroid carcinoma sections labelling Sumo 1 with purified ab133352 at dilution of 1:100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Western blot - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)

All lanes : Anti-Sumo 1 antibody [EP298] (ab133352) at 1/10000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
Lane 2 : MCF-7 (human breast carcinoma) whole cell lysate
Lane 3 : A549 (human lung carcinoma) whole cell lysate
Lane 4 : NIH/3T3 (mouse embryo) whole cell lysate
Lane 5 : C6 (rat glioma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Predicted band size: 11.5 kDa
Observed band size: 18-90 kDa
why is the actual band size different from the predicted?

This data was developed using ab133352, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)

All lanes : Anti-Sumo 1 antibody [EP298] (ab133352) at 1/1000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : HL60 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : NIH3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 11.5 kDa

This data was developed using ab133352, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)

This data was developed using ab133352, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human papillary cell thyroid gland carcinoma tissue labelling Sumo 1 with un-purified ab133352 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)

This data was developed using ab133352, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue labelling Sumo 1 with un-purified ab133352 at dilution of 1/100-1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)

All lanes : Anti-Sumo 1 antibody [EP298] (ab133352) at 1/1000 dilution

Lane 1 : Recombinant Human Sumo 1 protein (ab140417) at 0.01 µg
Lane 2 : Recombinant Human Sumo 2 protein (ab140420) at 0.01 µg
Lane 3 : Recombinant Human Sumo 3 protein (ab140414) at 0.01 µg
Lane 4 : Recombinant Human Sumo 4 protein (ab157025) at 0.1 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 11.5 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking buffer: 5% NFDM/TBST

This data was developed using ab133352, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)

This data was developed using ab133352, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Glioma tissue labelling Sumo 1 with un-purified ab133352 at dilution of 1/100-1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)

This data was developed using ab133352, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Urinary bladder transitional carcinoma tissue labelling Sumo 1 with un-purified ab133352 at dilution of 1/100-1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)

This data was developed using ab133352, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue labelling Sumo 1 with un-purified ab133352 at dilution of 1/100-1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)