Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP298] to Sumo 1 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Sumo 1 antibody [EP298] - BSA and Azide free
    See all Sumo 1 primary antibodies

  • Description

    Rabbit monoclonal [EP298] to Sumo 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248491 is the carrier-free version of ab133352. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248491 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)
    Western blot - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)

    This data was developed using ab133352, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: Sumo 1 knockout HAP1 cell lysate (20 µg) 

    Lane 3: HeLa cell lysate (20 µg)

    Lane 4: MCF-7 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab133352 observed at 16 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab133352 was shown to recognize Sumo 1 when Sumo 1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Sumo 1 knockout samples were subjected to SDS-PAGE. ab133352 and ab8245 (loading control to GAPDH) were diluted 1/000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)

    This data was developed using ab133352, the same antibody clone in a different buffer formulation.

    Immunohistochemical staining of paraffin-embedded human thyroid carcinoma sections labelling Sumo 1 with purified ab133352 at dilution of 1:100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Western blot - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)
    Western blot - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)
    All lanes : Anti-Sumo 1 antibody [EP298] (ab133352) at 1/10000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
    Lane 2 : MCF-7 (human breast carcinoma) whole cell lysate
    Lane 3 : A549 (human lung carcinoma) whole cell lysate
    Lane 4 : NIH/3T3 (mouse embryo) whole cell lysate
    Lane 5 : C6 (rat glioma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 11.5 kDa
    Observed band size: 18-90 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab133352, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)
    Western blot - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)
    All lanes : Anti-Sumo 1 antibody [EP298] (ab133352) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : HL60 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : NIH3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 11.5 kDa



    This data was developed using ab133352, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)
    This data was developed using ab133352, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human papillary cell thyroid gland carcinoma tissue labelling Sumo 1 with un-purified ab133352 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)
    This data was developed using ab133352, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue labelling Sumo 1 with un-purified ab133352 at dilution of 1/100-1/250.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)
    Western blot - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)
    All lanes : Anti-Sumo 1 antibody [EP298] (ab133352) at 1/1000 dilution

    Lane 1 : Recombinant Human Sumo 1 protein (ab140417) at 0.01 µg
    Lane 2 : Recombinant Human Sumo 2 protein (ab140420) at 0.01 µg
    Lane 3 : Recombinant Human Sumo 3 protein (ab140414) at 0.01 µg
    Lane 4 : Recombinant Human Sumo 4 protein (ab157025) at 0.1 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 11.5 kDa
    Observed band size: 16 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking buffer: 5% NFDM/TBST

    This data was developed using ab133352, the same antibody clone in a different buffer formulation.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)
    This data was developed using ab133352, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Glioma tissue labelling Sumo 1 with un-purified ab133352 at dilution of 1/100-1/250.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)
    This data was developed using ab133352, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Urinary bladder transitional carcinoma tissue labelling Sumo 1 with un-purified ab133352 at dilution of 1/100-1/250.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)
    This data was developed using ab133352, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue labelling Sumo 1 with un-purified ab133352 at dilution of 1/100-1/250.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)
    Anti-Sumo 1 antibody [EP298] - BSA and Azide free (ab248491)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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