Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16096] to SULT2A1/ST2
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SULT2A1/ST2 antibody [EPR16096]
    See all SULT2A1/ST2 primary antibodies

  • Description

    Rabbit monoclonal [EPR16096] to SULT2A1/ST2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, Human fetal liver, Human adrenal gland, mouse spleen, rat kidney and rat liver lysates; Human adrenal tissue; HepG2 cells.

  • General notes

     

     

     This product was previously labelled as SULT2A1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
    Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
    All lanes : Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : Human adrenal gland lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
    Western blot - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
    All lanes : Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113) at 1/2000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : Rat kidney lysate
    Lane 3 : Rat liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)

    Immunohistochemical analysis of paraffin-embedded Human adrenal tissue labeling SULT2A1/ST2 with ab194113 at 1/4000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
    Immunocytochemistry/ Immunofluorescence - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labeling SULT2A1/ST2 with ab194113 at 1/50 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).

  • Flow Cytometry - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
    Flow Cytometry - Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 cells labeling SULT2A1/ST2 with ab194113 at 1/120 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)
    Anti-SULT2A1/ST2 antibody [EPR16096] (ab194113)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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