Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Strep Tag antibody [11A7] (ab252885)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [11A7] to Strep Tag
  • Suitable for: WB, ICC/IF, ELISA, IP
  • Reacts with: Species independent

Overview

  • Product name

    Anti-Strep Tag antibody [11A7]
    See all Strep Tag primary antibodies

  • Description

    Rat monoclonal [11A7] to Strep Tag

  • Host species

    Rat

  • Tested applications

    Suitable for: WB, ICC/IF, ELISA, IPmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Fusion protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Strep-tagged standard marker. ICC/IF: Strep tag-GFP transfected 293T cells. IP: HEK-293T transfected with GFP expression vector containing a strep tag.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    11A7

  • Isotype

    IgG2b

  • Light chain type

    kappa

  • Research areas

Images

  • Western blot - Anti-Strep Tag antibody [11A7] (ab252885)
    Western blot - Anti-Strep Tag antibody [11A7] (ab252885)
    Anti-Strep Tag antibody [11A7] (ab252885) at 0.19 µg/ml + strep-tagged standard marker at 3 µl

    Secondary
    Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 19 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Strep Tag antibody [11A7] (ab252885)
    Immunocytochemistry/ Immunofluorescence - Anti-Strep Tag antibody [11A7] (ab252885)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Strep tag-GFP transfected HEK-293T cells labelling Strep Tag with ab252885 at 1/250 dilution (3.808µg/ml), followed by ab150160 Goat Anti-rat IgG H&L (Alexa Fluor® 594) antibody at 1/1000 (2µg/ml) dilution (Green). Confocal image showing nuclear and cytoplasmic staining in HEK-293T cells transfected with GFP-tagged GluN1 zeta-1 expression vector. The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150160 Goat Anti-rat IgG H&L (Alexa Fluor® 594) at 1/1000 (2µg/ml) dilution.

  • Immunoprecipitation - Anti-Strep Tag antibody [11A7] (ab252885)
    Immunoprecipitation - Anti-Strep Tag antibody [11A7] (ab252885)

    Strep Tag was immunoprecipitated from HEK-293T transfected with GFP expression vector containing a strep tag whole cell lysate with ab252885 at 1/30 dilution (31.733µg/ml) (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252885 at 1/1000 dilution. Goat Anti-rat IgG (H+L), HRP) (ab205720) was used at 1/10000 dilution.

    Lane 1: HEK-293T transfected with GFP expression vector containing a strep tag whole cell lysate 10µg.

    Lane 2: ab252885 IP in HEK-293T transfected with GFP expression vector containing a strep tag whole cell lysate.

    Lane 3: Rat monoclonal IgG2b instead of ab252885 in HEK-293T transfected with GFP expression vector containing a strep tag whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

     

  • ELISA - Anti-Strep Tag antibody [11A7] (ab252885)
    ELISA - Anti-Strep Tag antibody [11A7] (ab252885)

    ELISA - Anti-Strep Tag antibody [11A7] (ab252885).

    ab252885 used at 0-5000 ng/ml.

    Antigen (Strep tag) used at 1000 ng/ml.

    Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at a 1/1000 dilution.

  • Anti-Strep Tag antibody [11A7] (ab252885)
    Anti-Strep Tag antibody [11A7] (ab252885)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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