Anti-Bcl-2 (phospho S70) antibody (ab28819)
Key features and details
- Rabbit polyclonal to Bcl-2 (phospho S70)
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
-
Product name
Anti-Bcl-2 (phospho S70) antibody
See all Bcl-2 primary antibodies -
Description
Rabbit polyclonal to Bcl-2 (phospho S70) -
Host species
Rabbit -
Specificity
ab28819 recognises Phospho-BCL-2(Ser70). -
Tested Applications & Species
See all applications and species dataApplication Species WB HumanAfrican green monkey
-
Immunogen
Synthetic peptide corresponding to Human Bcl-2.
Database link: P10415
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab28819 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Bcl-2 (phospho S70) antibody (ab28819)
Western blot analysis of lysates from HeLa cells treated with LPS (40nM, 30mins), K562 cells treated with calyculin A (50ng/ml, 30mins) and COS-7 cells treated with H2O2 (1ng/ml, 15mins), using BCL-2 (Phospho-Ser70) Antibody. The lane on the right is blocked with the phospho peptide.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 (phospho S70) antibody (ab28819)Immunohistochemical analysis of paraffin-embedded breast carcinoma, using anti-Phospho-BCL2(Ser70) antibody (ab28819). Left: Untreated; Right: Treated with synthesized phosphopeptide.
