Anti-Staphylococcus aureus Enterotoxins A + B + TSST-1 antibody (ab190337)
Key features and details
- Rabbit polyclonal to Staphylococcus aureus Enterotoxins A + B + TSST-1
- Suitable for: Sandwich ELISA, WB, ELISA
- Reacts with: Staphylococcus aureus
- Isotype: IgG
Overview
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Product name
Anti-Staphylococcus aureus Enterotoxins A + B + TSST-1 antibody -
Description
Rabbit polyclonal to Staphylococcus aureus Enterotoxins A + B + TSST-1 -
Host species
Rabbit -
Specificity
ab190337 was designed to broadly react with a number of toxins produced by Staphylococcus aureus strains. Those confirmed include SEA, SEB, SEC, TSST-1. This antibody does not detect alpha-hemolysin, nor LukS and LukF. -
Tested applications
Suitable for: Sandwich ELISA, WB, ELISAmore details -
Species reactivity
Reacts with: Staphylococcus aureus -
Immunogen
Chemical/ Small Molecule corresponding to Staphylococcus aureus Enterotoxins A + B + TSST-1.
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Positive control
- Staphylococcal Superantigens: Enterotoxin B (SEB), Enterotoxin A (SEA), and Toxic Shock Syndrome Toxin (TSST-1).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 100% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ELISA - Anti-Staphylococcus aureus Enterotoxins A + B + TSST-1 antibody (ab190337)
Various Staphylococcal toxins were coated to a plate at a concentration of 1 μg/mL in PBS. ab190337 was serially diluted semi-log from 2 μg/mL and incubated with the toxin coated plate. Washed plates were detected with anti-rabbit HRP conjugate and TMB substrate. OD650 was reported above.
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Western blot - Anti-Staphylococcus aureus Enterotoxins A + B + TSST-1 antibody (ab190337)All lanes : Anti-Staphylococcus aureus Enterotoxins A + B + TSST-1 antibody (ab190337) at 1 µg/ml
Lane 1 : Enterotoxin B (SEB) at 0.05 µg
Lane 2 : Enterotoxin A (SEA) at 0.05 µg
Lane 3 : Enterotoxin C (SEC-1) at 0.05 µg
Lane 4 : Toxic Shock Syndrome Toxin (TSST-1) at 0.05 µg
Lane 5 : 24 hour culture supernatant of an SEB producing Staphylocccus aureus strain at 15 µl
Lane 6 : Media without culture growth at 15 µl
Lane 7 : LukS-PV at 0.05 µg
Lane 8 : LukF-PV at 0.05 µg
Secondary
All lanes : Anti-rabbit HRP conjugate
Observed band size: 27-30 kDa why is the actual band size different from the predicted?Bands visualized using TMB membrane substrate.