Anti-SQSTM1 / p62 antibody (ab227207)
Key features and details
- Rabbit polyclonal to SQSTM1 / p62
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SQSTM1 / p62 antibody
See all SQSTM1 / p62 primary antibodies -
Description
Rabbit polyclonal to SQSTM1 / p62 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment within Human SQSTM1/ p62 (internal sequence). The exact sequence is proprietary.
Database link: Q13501 -
Positive control
- WB: HepG2 whole cell extract; Raji, K562, HL-60 and NCI-H929 whole cell lysates. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SQSTM1 / p62 antibody (ab227207)All lanes : Anti-SQSTM1 / p62 antibody (ab227207) at 1/7000 dilution
Lane 1 : Non-transfected HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lane 2 : SQSTM1 / p62 shRNA transfected HeLa whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa10% SDS-PAGE gel.
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Western blot - Anti-SQSTM1 / p62 antibody (ab227207)All lanes : Anti-SQSTM1 / p62 antibody (ab227207) at 1/10000 dilution
Lane 1 : Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lane 2 : HepG2, treated with 3 µM thapsigargin for 12 hours, whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa10% SDS-PAGE gel.
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Western blot - Anti-SQSTM1 / p62 antibody (ab227207)All lanes : Anti-SQSTM1 / p62 antibody (ab227207) at 1/10000 dilution
Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Lane 4 : NCI-H929 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody (ab227207)Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SQSTM1 / p62 (green) using ab227207 at 1/500 dilution in ICC/IF.
Alpha-Tubulin was detected with an anti-alpha-Tubulin antibody at 1 /2000 dilution (red).
