Anti-SPC25 antibody (ab20679)
Key features and details
- Rabbit polyclonal to SPC25
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SPC25 antibody
See all SPC25 primary antibodies -
Description
Rabbit polyclonal to SPC25 -
Host species
Rabbit -
Specificity
This antibody recognizes a protein of approximately 31kDa in HeLa cell lysate that is blocked by the addition of the immunizing peptide. This is larger than the expected size of 25kDa and we cannot explain why the detected protein is larger than expected. Several larger bands are detected which are not blocked at all by the addition of the immunizing peptide. SPC25 may be better detected in lysates of nocodazole-arrested HeLa cells.
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Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Spc25.
Read Abcam's proprietary immunogen policy (Peptide available as ab23408.)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20679 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseApplication Abreviews Notes WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 26 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 26 kDa).Target
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Function
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. -
Sequence similarities
Belongs to the SPC25 family. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. - Information by UniProt
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Database links
- Entrez Gene: 57405 Human
- Entrez Gene: 66442 Mouse
- Entrez Gene: 295661 Rat
- Omim: 609395 Human
- SwissProt: Q9HBM1 Human
- SwissProt: Q3UA16 Mouse
- SwissProt: Q9D021 Mouse
- SwissProt: Q9D1K6 Mouse
see all -
Alternative names
- AD024 antibody
- hSpc25 antibody
- Kinetochore protein Spc25 antibody
see all
Images
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Western blot - Anti-SPC25 antibody (ab20679)All lanes : Anti-SPC25 antibody (ab20679) at 1 µg/ml
Lane 1 : NIH/3T3 (Mouse) Whole Cell Lysate (ab52956)
Lane 2 : Human ovary tissue lysate - total protein (ab30222)
Lane 3 : Human pancreas tissue lysate - total protein (ab29816)
Lane 4 : Pancreas (Mouse) Tissue Lysate (ab55116)
Lane 5 : Ovary (Mouse) Tissue Lysate (ab35808)
Lane 6 : Ovary (Rat) Tissue Lysate (ab65552)
Lane 7 : Pancreas (Rat) Tissue Lysate (ab4036)
Lane 8 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa
Datasheets and documents
References (3)
ab20679 has been referenced in 3 publications.
- Chen J et al. SPC25 upregulation increases cancer stem cell properties in non-small cell lung adenocarcinoma cells and independently predicts poor survival. Biomed Pharmacother 100:233-239 (2018). WB . PubMed: 29432994
- Villegas E et al. Plk2 regulates mitotic spindle orientation and mammary gland development. Development 141:1562-71 (2014). WB . PubMed: 24598160
- Sun SC et al. Perturbation of Spc25 expression affects meiotic spindle organization, chromosome alignment and spindle assembly checkpoint in mouse oocytes. Cell Cycle 9:4552-9 (2010). ICC/IF ; Mouse . PubMed: 21084868
Images
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Western blot - Anti-SPC25 antibody (ab20679)All lanes : Anti-SPC25 antibody (ab20679) at 1 µg/ml
Lane 1 : NIH/3T3 (Mouse) Whole Cell Lysate (ab52956)
Lane 2 : Human ovary tissue lysate - total protein (ab30222)
Lane 3 : Human pancreas tissue lysate - total protein (ab29816)
Lane 4 : Pancreas (Mouse) Tissue Lysate (ab55116)
Lane 5 : Ovary (Mouse) Tissue Lysate (ab35808)
Lane 6 : Ovary (Rat) Tissue Lysate (ab65552)
Lane 7 : Pancreas (Rat) Tissue Lysate (ab4036)
Lane 8 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa
