Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SOX9 antibody [EPR14335] - BSA and Azide free (ab220812)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14335] to SOX9 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SOX9 antibody [EPR14335] - BSA and Azide free
    See all SOX9 primary antibodies

  • Description

    Rabbit monoclonal [EPR14335] to SOX9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • SW480 and PC3 cell lysates; Human colon, Rat colon and Mouse colon tissues; SW480 cells; PC3 cells.

  • General notes

    Ab220812 is the carrier-free version of ab185230. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab220812 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] - BSA and Azide free (ab220812)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] - BSA and Azide free (ab220812)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling SOX9 with ab185230 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185230).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-SOX9 antibody [EPR14335] - BSA and Azide free (ab220812)
    Immunoprecipitation - Anti-SOX9 antibody [EPR14335] - BSA and Azide free (ab220812)

    ab185230 at 1/60 immunoprecipitating SOX9 in SW480 (human colorectal adenocarcinoma) whole cell lysate observed at 56 and 75 KDa (lanes 1 and 2).

    The expression pattern observed is consistent with the literature (PMID: 10682837) with an additional off-target band at 46 kD.

    Lane 1 (input): SW480 whole cell lysate 10μg

    Lane 2 (+): SW480 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab185230 in SW480 whole cell lysate

    For western blotting, ab185230 (1/1000) was used as the primary antibody and ab131366 VeriBlot for IP (HRP) was used for detection at 1/1000 dilution.

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185230).

  • Flow Cytometry - Anti-SOX9 antibody [EPR14335] - BSA and Azide free (ab220812)
    Flow Cytometry - Anti-SOX9 antibody [EPR14335] - BSA and Azide free (ab220812)

    Flow Cytometric analysis of PC3 cells (2% paraformaldehyde-fixed) labeling SOX9 with ab185230 at 1/190 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185230).

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335] - BSA and Azide free (ab220812)
    Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335] - BSA and Azide free (ab220812)

    Immunofluorescent analysis of SW480 cells (4% paraformaldehyd-fixed) labeling SOX9 with ab185230 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185230).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] - BSA and Azide free (ab220812)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] - BSA and Azide free (ab220812)

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling SOX9 with ab185230 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185230).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] - BSA and Azide free (ab220812)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335] - BSA and Azide free (ab220812)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SOX9 with ab185230 at 1/2000 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185230).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-SOX9 antibody [EPR14335] - BSA and Azide free (ab220812)
    Anti-SOX9 antibody [EPR14335] - BSA and Azide free (ab220812)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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